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However, L\u2010type voltage\u2010dependent Ca<jats:sup>2+<\/jats:sup> channel (LVDCC) blockers were recently demonstrated to significantly reduce NTBI uptake by cardiomyocytes, implying that LVDCC plays a dominant role in NTBI uptake by cardiomyocytes under iron\u2010overloaded conditions. These findings led us to hypothesize that the LVDCC blocker\u2010induced reduction in NTBI uptake might result not only from the inhibition of LVDCC\u2010mediated NTBI uptake but also from the suppression of DMT1\u2010mediated NTBI uptake. We therefore investigated the effects of the LVDCC blocker verapamil on NTBI uptake as well as DMT1 expression in H9C2 cells by the measurement of radio\u2010labeled <jats:sup>55<\/jats:sup>Fe(II), reverse transcription polymerase chain reaction (RT\u2010PCR) and western blot analysis. We demonstrated that verapamil induced a significant reduction in NTBI uptake by H9C2 cells but also unexpectedly a remarkable increase rather than decrease in the expression of DMT1 mRNA and protein in H9C2 cells. Our findings imply that the verapamil\u2010induced reduction in NTBI uptake by H9C2 cells is not associated with DMT1 and also indicate that verapamil stimulates rather than inhibits DMT1 expression and DMT1\u2010mediated iron uptake by heart cells.<\/jats:p>","DOI":"10.1111\/fcp.12793","type":"journal-article","created":{"date-parts":[[2022,4,30]],"date-time":"2022-04-30T01:53:25Z","timestamp":1651283605000},"page":"985-991","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Verapamil downregulates iron uptake and upregulates divalent metal transporter 1 expression in H9C2 cardiomyocytes"],"prefix":"10.1111","volume":"36","author":[{"given":"Li\u2010Rong","family":"Jiang","sequence":"first","affiliation":[{"name":"Laboratory of Neuropharmacology Fudan University School of Pharmacy  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng\u2010Qi","family":"Shen","sequence":"additional","affiliation":[{"name":"Institute of Translational and Precision Medicine Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Xin","family":"Bao","sequence":"additional","affiliation":[{"name":"Research Center for Medicine and Biology Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6399-938X","authenticated-orcid":false,"given":"Zhong\u2010Ming","family":"Qian","sequence":"additional","affiliation":[{"name":"Laboratory of Neuropharmacology Fudan University School of Pharmacy  Shanghai China"},{"name":"Institute of Translational and Precision Medicine Nantong University  Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167\u20104889(95)00098\u2010X"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.10284"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1992.262.2.R220"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.3.1148"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/10245330500093419"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304\u20104165(02)00497\u2010X"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102003\u201003\u20100807"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079\u20106107(97)00009\u20106"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2015.00019"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm920"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000124229.40424.80"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00153.2007"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1542"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00060"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102010\u201003\u2010274738"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.84.11.1302"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/fcp.12624"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/fcp.12562"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2008.07.014"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2007.10.013"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00221\u2010006\u20100823\u2010x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2010.06.013"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035\u2010014\u20108847\u2010x"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.105.017756"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167\u20104889(95)00175\u20101"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006\u20102952(90)90492\u20104"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005\u20102736(88)90374\u20104"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304\u20104165(91)90215\u20103"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167\u20104889(92)90163\u20106"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.54.4.561"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109\u2010005\u20100029\u2010x"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0143\u20104160(02)00240\u20103"}],"container-title":["Fundamental &amp; 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