{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T22:59:14Z","timestamp":1791673154478,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,6,24]],"date-time":"2023-06-24T00:00:00Z","timestamp":1687564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Fundamemntal Clinical Pharma"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Tylophora indica<\/jats:italic> (Burm. f.) Merr is a climbing perennial plant reported in Indian traditional system of medicine for its use in allergy and asthma. However, only few scientific studies have been performed in the past to validate its antiasthmatic potential.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The present study deals with investigation of airway smooth muscle relaxant and antiasthmatic potential of extract and subsequent fractions prepared from <jats:styled-content style=\"fixed-case\"><jats:italic>T.\u00a0indica<\/jats:italic><\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The most active fraction of <jats:styled-content style=\"fixed-case\"><jats:italic>T.\u00a0indica<\/jats:italic><\/jats:styled-content> leaves selected through bio\u2010guided activity was subjected to liquid chromatography\u2010mass spectrometry (LC\u2013MS) analysis for chemical profiling. The binding affinity of identified compounds in fraction towards M<jats:sub>3<\/jats:sub> and H<jats:sub>1<\/jats:sub> receptors was determined by molecular docking study. F\u20102 (chloroform fraction prepared from methanolic extract of <jats:styled-content style=\"fixed-case\"><jats:italic>T.\u00a0indica<\/jats:italic><\/jats:styled-content> leaves) was examined for its smooth muscle relaxant properties using isolated trachea of guinea\u2010pig. Further, F\u20102 was evaluated through in vivo studies employing ovalbumin\u2010induced asthma model in guinea\u2010pigs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>F\u20102 was found most effective in bioassay\u2010guided fractionation. Characterization by LC\u2013MS analysis revealed presence of five major bioactive compounds in F\u20102 that showed good docking interactions with M<jats:sub>3<\/jats:sub> and H<jats:sub>1<\/jats:sub> receptors. The ex vivo study demonstrated that F\u20102 could significantly relax tracheal rings via targeting multiple signalling pathways videlicet, namely, noncompetitive antagonism of the histamine and muscarinic receptors, \u03b22\u2010adrenergic stimulation and activation of soluble guanylyl cyclase. In in vivo studies, F\u20102 ameliorated airway hyperresponsiveness and decreased broncho alveolar lavage fluid (BALF) levels of inflammatory cytokines and immunoglobulin E (IgE).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results confirm the traditional use of <jats:styled-content style=\"fixed-case\"><jats:italic>T.\u00a0indica<\/jats:italic><\/jats:styled-content> as an antiasthmatic agent which are evidenced through ex vivo, in silico and in vivo studies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/fcp.12927","type":"journal-article","created":{"date-parts":[[2023,6,25]],"date-time":"2023-06-25T01:38:12Z","timestamp":1687657092000},"page":"1153-1169","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<i>Tylophora indica<\/i> (Burm. f.) Merr alleviates tracheal smooth muscle hyperresponsiveness in ovalbumin\u2010induced allergic\u2010asthma model in guinea\u2010pigs: Evidences from ex vivo, in silico and in vivo studies"],"prefix":"10.1111","volume":"37","author":[{"given":"Ritika","family":"Gururani","sequence":"first","affiliation":[{"name":"Department of Pharmacy Banasthali Vidyapith  Banasthali India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saraswati","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Banasthali Vidyapith  Banasthali India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akansha","family":"Bisht","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Banasthali Vidyapith  Banasthali India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Smita","family":"Jain","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Banasthali Vidyapith  Banasthali India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarvesh","family":"Paliwal","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Banasthali Vidyapith  Banasthali India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1524-7714","authenticated-orcid":false,"given":"Jaya","family":"Dwivedi","sequence":"additional","affiliation":[{"name":"Department of Chemistry Banasthali Vidyapith  Banasthali India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2639-7096","authenticated-orcid":false,"given":"Swapnil","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Banasthali Vidyapith  Banasthali India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,6,24]]},"reference":[{"key":"e_1_2_11_2_1","unstructured":"Network GA.The Global Asthma Report 2014;2014.http:\/\/globalasthmareport.org\/burden\/burden.php"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.116300"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110616"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X15500615"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.PHYMED.2018.12.004"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X12500930"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/PTR.4791"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114361"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JEP.2020.113122"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20102006\u2010960136"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.31557\/APJCP.2018.19.11.3065"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.PHYMED.2018.05.004"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.112643"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JEP.2019.112014"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12906\u2010017\u20101766\u20104"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.15562\/phytomedicine.2019.97"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ddr.21935"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.112162"},{"key":"e_1_2_11_20_1","unstructured":"GuidelinesO The FOR Of T. 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