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The search for natural or synthetic compounds with antibacterial properties capable of reversing the resistance of <jats:italic>S. aureus<\/jats:italic> is the main challenge to be overcome today. Natural products such as chalcones are substances present in the secondary metabolism of plants, presenting important biological activities such as antitumor, antidiabetic, and antimicrobial activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>In this context, the aim of this work was to synthesize the chalcone (2<jats:italic>E<\/jats:italic>)\u20101\u2010(3'\u2010aminophenyl)\u20103\u2010(4\u2010dimethylaminophenyl)\u2010prop\u20102\u2010en\u20101\u2010one with nomenclature CMADMA, confirm its structure by nuclear magnetic resonance (NMR), and evaluate its antibacterial properties.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The synthesis methodology used was that of Claisen\u2010Schmidt, and spectroscopic characterization was performed by NMR. For microbiological assays, the broth microdilution methodology was adopted in order to analyze the antibacterial potential of chalcones and to analyze their ability to act as a possible inhibitor of \u03b2\u2010lactamase and efflux pump resistance mechanisms, present in S. aureus strain K4100.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results obtained show that CMADMA does not show direct antibacterial activity, expressing a MIC of \u22651024 \u03bcg\/mL, or on the enzymatic mechanism of \u03b2\u2010lactamase; however, when associated with ethidium bromide in efflux pump inhibition assays, CMADMA showed promising activity by reducing the MIC of the bromide from 64 to 32 \u03bcg\/mL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We conclude that the chalcone synthesized in this study is a promising substance to combat bacterial resistance, possibly acting in the inhibition of the QacC efflux pump present in <jats:italic>S. aureus<\/jats:italic> strain K4100, as evidenced by the reduction in the MIC of ethidium bromide.<\/jats:p><\/jats:sec>","DOI":"10.1111\/fcp.12938","type":"journal-article","created":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T10:58:50Z","timestamp":1690455530000},"page":"60-71","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Synthesis, spectroscopic characterization, and antibacterial activity of chalcone (2<i>E<\/i>)\u20101\u2010(3\u2032\u2010aminophenyl)\u20103\u2010(4\u2010dimethylaminophenyl)\u2010prop\u20102\u2010en\u20101\u2010one against multiresistant <scp><i>Staphylococcus aureus<\/i><\/scp> carrier of efflux pump mechanisms and \u03b2\u2010lactamase"],"prefix":"10.1111","volume":"38","author":[{"given":"Larissa","family":"da Silva","sequence":"first","affiliation":[{"name":"Laboratory of Semi\u2010Arid Bioprospecting (LABSEMA), Department of Biological Chemistry URCA  Crato 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