{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T02:22:35Z","timestamp":1773109355422,"version":"3.50.1"},"reference-count":72,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T00:00:00Z","timestamp":1715558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Fundamemntal Clinical Pharma"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Candida<\/jats:italic> spp. is an opportunistic pathogen capable of causing superficial to invasive infections. Morphological transition is one of the main virulence factors of this genus and, therefore, is an important variable to be considered in pharmacological interventions. Riparins I, II, III, and IV are alkamide\u2010type alkaloids extracted from the unripe fruit of <jats:italic>Aniba riparia<\/jats:italic>, whose remarkable pharmacological properties were previously demonstrated. This work aimed to evaluate in silico and in vitro the inhibitory effects of Riparins on the morphological transition of <jats:styled-content style=\"fixed-case\"><jats:italic>Candida albicans<\/jats:italic><\/jats:styled-content>, <jats:styled-content style=\"fixed-case\"><jats:italic>Candida tropicalis<\/jats:italic><\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\"><jats:italic>Candida krusei<\/jats:italic><\/jats:styled-content>. Molecular docking was applied to analyze the inhibitory effects of riparins against proteins such as <jats:italic>N<\/jats:italic>\u2010acetylglucosamine, CYP\u201051, and protein kinase A (PKA) using the Ramachandran plot. The ligands were prepared by MarvinSketch and Spartan software version 14.0, and MolDock Score and Rerank Score were used to analyze the affinity of the compounds. In vitro analyses were performed by culturing the strains in humid chambers in the presence of riparins or fluconazole (FCZ). The morphology was observed through optical microscopy, and the size of the hyphae was determined using the ToupView software. In silico analysis demonstrated that all riparins are likely to interact with the molecular targets: GlcNAc (&gt;50%), PKA (&gt;60%), and CYP\u201051 (&gt;70%). Accordingly, in vitro analysis showed that these compounds significantly inhibited the morphological transition of all <jats:italic>Candida<\/jats:italic> strains. In conclusion, this study demonstrated that riparins inhibit <jats:italic>Candida<\/jats:italic> morphological transition and, therefore, can be used to overcome the pathogenicity of this genus.<\/jats:p>","DOI":"10.1111\/fcp.13007","type":"journal-article","created":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T08:54:52Z","timestamp":1715590492000},"page":"946-957","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Inhibition of the morphological transition of <i>Candida<\/i> spp. by riparins I\u2013IV"],"prefix":"10.1111","volume":"38","author":[{"given":"Maria Lucilene","family":"Queiroz da Silva","sequence":"first","affiliation":[{"name":"Departamento de Qu\u00edmica Biol\u00f3gica Universidade Regional do Cariri (URCA)  Crato Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nat\u00e1lia","family":"Ferreira de Sousa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de 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