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Bladder and vas deferens (prostatic and epididymal portions) tissues were mounted in 10\u2009mL of organ baths. Relaxation responses to anandamide were recorded at 3 and 10\u2009\u03bcM concentrations. After the rest period, the procedures were repeated in the presence of cannabinoid (CB) and vanilloid receptor antagonists, various potassium channel blockers, cyclo\u2010oxygenase, and nitric oxide synthase inhibitors. In different tissues to investigate the Ca<jats:sup>2+<\/jats:sup>\u2010channel antagonistic effect of anandamide, concentration\u2013response curves to CaCl<jats:sub>2<\/jats:sub> were obtained in the absence and presence of anandamide.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Anandamide caused a significant relaxation response in the bladder and epididymal vas deferens tissues, but not in the prostatic portion. The effect of anandamide was antagonized in the presence of the CB<jats:sub>1<\/jats:sub> antagonist AM251 or the non\u2010selective potassium channel blocker tetraethylammonium in bladder tissue. In the epididymal vas deferens, anandamide significantly inhibited the calcium contraction responses, especially at high concentrations. The CB<jats:sub>2<\/jats:sub> antagonist AM630 reversed this inhibition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results show that anandamide has a direct relaxant effect on the isolated rat bladder and epididymal vas deferens. Anandamide triggers different mechanisms in different types of tissues, and further studies are needed to elucidate the mechanism of action of anandamide.<\/jats:p><\/jats:sec>","DOI":"10.1111\/fcp.13008","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T06:53:02Z","timestamp":1715151182000},"page":"967-977","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The relaxation effect of endocannabinoid anandamide on isolated rat bladder and vas deferens tissues and possible mechanisms"],"prefix":"10.1111","volume":"38","author":[{"given":"Yagmur","family":"Okcay","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Gulhane Faculty of Pharmacy University of Health Sciences T\u00fcrkiye  Etlik\/Kecioren\/Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cagil","family":"Onal Sis","sequence":"additional","affiliation":[{"name":"Department of 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