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In this study, we investigated whether the vasodilator effects occur in coronary vessels and explored whether hyperpolarization is involved in the underlying mechanism of NPP\u2010induced smooth muscle relaxation. The relaxing responses were studied in isolated preparations of the left anterior descending coronary (ADC) and the septal coronary (SC) arteries, which had been previously maintained under sustained contraction induced by the thromboxane A\n                    <jats:sub>2<\/jats:sub>\n                    analogue U\u201046619. Administered cumulatively, NPP elicited concentration\u2010dependent vasorelaxation with similar potency in both vessels. The relaxant effect remained unaffected by the nitric oxide synthase inhibitor L\u2010NAME, the protein kinase C inhibitor bisindolylmaleimide IV and the Rho\u2010associated protein kinase inhibitor Y\u201027632. However, it was significantly diminished by the adenylyl cyclase inhibitor MDL\u201012,330A, the guanylyl cyclase inhibitor ODQ, as well as the K\n                    <jats:sup>+<\/jats:sup>\n                    channel inhibitors tetraethylammonium and CsCl. In ADC preparations impaled with intracellular micropipettes, NPP hyperpolarized the vascular preparation. When the isolated preparation was precontracted by 5\u2010hydroxytryptamine or 80\u2009mM KCl, NPP\u2010induced relaxation with lower pharmacological potency compared to the vessels contracted by U\u201046619. In conclusion, NPP exhibits vasorelaxant effects on rat coronary arteries, likely involving pathways that include cyclic nucleotide production and membrane hyperpolarization.\n                  <\/jats:p>","DOI":"10.1111\/fcp.13038","type":"journal-article","created":{"date-parts":[[2024,10,5]],"date-time":"2024-10-05T02:33:35Z","timestamp":1728095615000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In vitro evidence that the vasorelaxant effects of 2\u2010nitro\u20101\u2010phenyl\u20101\u2010propanol on rat coronary arteries involve cyclic nucleotide pathways"],"prefix":"10.1111","volume":"39","author":[{"given":"Alfredo Augusto","family":"Vasconcelos\u2010Silva","sequence":"first","affiliation":[{"name":"Department of Physiology and Pharmacology, School of Medicine Federal University of Cear\u00e1  Fortaleza Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suliana 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