{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T14:16:54Z","timestamp":1773929814543,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T00:00:00Z","timestamp":1735862400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T00:00:00Z","timestamp":1735862400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100014812","name":"Nordic Pharma Group","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014812","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Fundamemntal Clinical Pharma"],"published-print":{"date-parts":[[2025,2]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Methotrexate (MTX) is the first\u2010line treatment for Rheumatoid Arthritis (RA), yet 30%\u201350% of RA patients develop resistance to MTX, which can manifest several years after treatment initiation.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n This study investigates the relationship between erythrocyte methotrexate polyglutamates (MTX\u2010PGs) subtype concentrations and clinical disease activity in RA patients undergoing long\u2010term MTX treatment.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n In this cross\u2010sectional study, patients on a stable dose of subcutaneous MTX for several years were included. The study protocol was registered in the European Medicines Agency's clinical trials register (n\u00b02017\u2010004348\u201039). Patients were classified as either in clinical remission (DAS28 <2.6) or having active disease (DAS28 >3.2). Erythrocyte MTX\u2010PGs concentrations were measured using liquid chromatography coupled with mass spectrometry. Multivariate logistic regression analysis assessed the probability of remission status based on MTX\u2010PG3 concentrations.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The study included 34 patients with active RA and 25 in remission. The remission group had a median MTX treatment duration of 6.4\u00a0years compared to 2.6\u00a0years for the active group (\n p<\/jats:italic>\n \u2009=\u20090.001). Patients in remission had a longer median disease duration (\n p<\/jats:italic>\n \u2009=\u20090.02) and a lower Body Mass Index (BMI) (\n p<\/jats:italic>\n \u2009=\u20090.03) than those with active RA. A positive correlation was found between remission status and high MTX\u2010PG3 concentrations in patients with a BMI <25\u2009kg\/m\n 2<\/jats:sup>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Erythrocyte MTX\u2010PG3 concentrations may serve as a marker for RA activity after prolonged treatment. However, BMI could limit their utility as a biomarker.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/fcp.13050","type":"journal-article","created":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T03:54:37Z","timestamp":1735876477000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Long\u2010term evaluation of rheumatoid arthritis activity with erythrocyte methotrexate\u2010polyglutamate 3"],"prefix":"10.1111","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7348-418X","authenticated-orcid":false,"given":"Jean","family":"Escal","sequence":"first","affiliation":[{"name":"CHU Saint\u2010Etienne, Laboratoire de Pharmacologie et Toxicologie, Mines Saint\u2010Etienne, INSERM, SAINBIOSE U1059 Universit\u00e9 Jean Monnet Saint\u2010\u00c9tienne Saint\u2010Etienne 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