{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T08:05:58Z","timestamp":1784793958291,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100019486","name":"Biocodex Microbiota Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019486","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Fundamemntal Clinical Pharma"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>There is a complex link between environmental factors, anxiety levels, and ethanol consumption. Social conditions such as loneliness, peer influence, and housing environment shape drinking behaviors and mental health outcomes. The gut microbiota, in the context of the gut\u2010brain axis, may act as a biomarker.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study investigates how different housing conditions impact anxiety\u2010like behavior, ethanol consumption, and gut microbiota in adult male mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Adult male C57BL\/6JRj mice were housed under isolated, semi\u2010isolated, or group\u2010housed conditions. Anxiety\u2010like behavior was assessed using the open field (OF), elevated plus maze (EPM), and novelty\u2010suppressed feeding (NSF) tests. Ethanol intake was evaluated using a 4\u2010week intermittent two\u2010bottle choice paradigm. Fecal samples were collected and analyzed by 16S rRNA sequencing to characterize gut microbiota. Behavioral, drinking, and microbiota data were analyzed using ANOVA\u2010based statistics and QIIME2 pipelines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Semi\u2010isolated mice displayed anxiety\u2010like behavior only in the NSF test. Gut microbiota analysis revealed no significant changes in global diversity. Semi\u2010isolated mice exhibited a specific reduction in the Prevotellaceae, particularly\n                      <jats:italic>Prevotella UCG_001<\/jats:italic>\n                      . Housing conditions also influenced ethanol intake: isolated mice exhibited a low initial preference that increased over time, whereas semi\u2010isolated mice showed a high initial preference followed by avoidance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Housing conditions strongly shape voluntary ethanol consumption. Semi\u2010isolation was associated with elevated initial alcohol preference and anxiety\u2010like behavior only in the NSF test, while isolation led to a gradual escalation of ethanol intake. These findings highlight the importance of housing environment and anxiety\u2010related factors in alcohol\u2010related preclinical research.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/fcp.70093","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T00:01:12Z","timestamp":1779753672000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Semi\u2010Isolation Housing Paradigm Affects Anxiety, Ethanol Intake, and the Gut Microbiota in Adult Male C57BL\/6JRj Mice"],"prefix":"10.1111","volume":"40","author":[{"given":"Anish","family":"Nusrat","sequence":"first","affiliation":[{"name":"Centre de Recherche en Epid\u00e9miologie et Sant\u00e9 des Populations (CESP), UMR 1018, CESP\u2010INSERM, Team Moods, Facult\u00e9 de Pharmacie, B\u00e2timent Henri Moissan Universit\u00e9 Paris\u2010Saclay, UVSQ  Orsay 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