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We previously demonstrated that hepatocyte\u2010specific deletion of <jats:italic><jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>rf1<\/jats:italic> in mice resulted in spontaneous apoptosis, inflammation, and development of liver tumors. Here, we showed that both fibroblasts derived from <jats:italic><jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>rf1<\/jats:italic> null mouse embryos and fibroblasts expressing a conditional <jats:italic><jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>rf1<\/jats:italic> allele showed increased micronuclei and formation of abnormal nuclei. Lentiviral sh<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010mediated knockdown of <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>rf1 in <jats:styled-content style=\"fixed-case\">SAOS<\/jats:styled-content>\u20132 cells also resulted in increased micronuclei, abnormal mitosis and multi\u2010nucleated cells. Metaphase analyses showed increased aneuploidy in <jats:italic><jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>rf1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> embryonic fibroblasts. Nuclear defects in Nrf1\u2010deficient cells were associated with decreased expression of various genes encoding kinetochore and mitotic checkpoint proteins. Our findings suggest that <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>rf1 may play a role in maintaining genomic integrity, and that <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>rf1 dysregulation may induce tumorigenesis.<\/jats:p>","DOI":"10.1111\/febs.12005","type":"journal-article","created":{"date-parts":[[2012,9,12]],"date-time":"2012-09-12T16:15:39Z","timestamp":1347466539000},"page":"4121-4130","source":"Crossref","is-referenced-by-count":38,"title":["Deficiency in the nuclear\u2010related factor erythroid\u00a02 transcription factor (<scp>N<\/scp>rf1) leads to genetic instability"],"prefix":"10.1111","volume":"279","author":[{"given":"Diane H.","family":"Oh","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of California at Irvine CA 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