{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T23:16:47Z","timestamp":1781651807174,"version":"3.54.5"},"reference-count":23,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2012,11,12]],"date-time":"2012-11-12T00:00:00Z","timestamp":1352678400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:sec><jats:label\/><jats:p>Coherent glycolytic oscillations in <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>accharomyces\u00a0cerevisiae<\/jats:italic> are a multicellular property induced by addition of glucose to a starved cell population of sufficient density. However, initiation of oscillations requires an additional perturbation, usually addition of cyanide. The fate of cyanide during glycolytic oscillations has not previously been studied, and is the subject of the present paper. Using a cyanide electrode, a substantial decrease in cyanide concentration was observed. In the pH range 6\u20137, we found experimentally that the electrode behaves reasonably well, provided changes in pH are taken into account. To our knowledge, use of a cyanide electrode to study cyanide dynamics in living biological systems is new. Cyanide was found to enter starving yeast cells in only negligible amounts, and did not react significantly with glucose. Thus, cyanide consumption must be explained by reactions with glycolytic intermediates and evaporation. Evaporation and reaction with the signalling substance, extracellular acetaldehyde (<jats:styled-content style=\"fixed-case\">ACA<\/jats:styled-content><jats:sub><jats:italic>x<\/jats:italic><\/jats:sub>) only explains the observed cyanide removal if [<jats:styled-content style=\"fixed-case\">ACA<\/jats:styled-content><jats:sub><jats:italic>x<\/jats:italic><\/jats:sub>] is improbably high. Furthermore, differences in <jats:styled-content style=\"fixed-case\">NADH<\/jats:styled-content> traces upon cyanide addition before or after glucose addition strongly suggest that cyanide also reacts with intracellular carbonyl\u2010containing metabolites. We show that cyanide reacts with pyruvate (<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>yr) and dihydroxyacetone phosphate in addition to <jats:styled-content style=\"fixed-case\">ACA<\/jats:styled-content>, and estimate their rate constants. Our results strongly suggest that the major routes of cyanide removal during glycolysis are reactions with pyruvate and <jats:styled-content style=\"fixed-case\">ACA<\/jats:styled-content>. Cyanide removal by all carbonyl\u2010containing intermediates led to a lower mean [<jats:styled-content style=\"fixed-case\">ACA<\/jats:styled-content><jats:sub><jats:italic>x<\/jats:italic><\/jats:sub>], thereby increasing the amplitude of [<jats:styled-content style=\"fixed-case\">ACA<\/jats:styled-content><jats:sub><jats:italic>x<\/jats:italic><\/jats:sub>] oscillations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Database<\/jats:title><jats:p>The mathematical model described here has been submitted to the JWS Online Cellular Systems Modelling Database and can be accessed at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/jjj.biochem.sun.ac.za\/database\/hald\/index.html\">http:\/\/jjj.biochem.sun.ac.za\/database\/hald\/index.html<\/jats:ext-link> free of charge.<\/jats:p><\/jats:sec>","DOI":"10.1111\/febs.12030","type":"journal-article","created":{"date-parts":[[2012,10,16]],"date-time":"2012-10-16T16:11:20Z","timestamp":1350403880000},"page":"4410-4420","source":"Crossref","is-referenced-by-count":13,"title":["Influence of cyanide on diauxic oscillations in yeast"],"prefix":"10.1111","volume":"279","author":[{"given":"Bj\u00f8rn O.","family":"Hald","sequence":"first","affiliation":[{"name":"Department of Biomedical Health Sciences University of Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslava","family":"Smrcinova","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Preben G.","family":"S\u00f8rensen","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Copenhagen 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