{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T15:29:58Z","timestamp":1773588598278,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2012,11,22]],"date-time":"2012-11-22T00:00:00Z","timestamp":1353542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:p>Pre\u2010eclampsia is thought to be a systemic disease of maternal endothelial cell dysfunctions. mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s regulate various basic biological functions in cells, including stem cells. Mesenchymal stem cells exist in almost all tissues and are the key cellular source for tissue repair and regeneration. Our aims are to investigate whether mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s regulate <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s in fetal\u2013maternal interfaces to influence the pathogenesis of pre\u2010eclampsia. The differential expression of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in decidua\u2010derived mesenchymal stem cells of all patients with severe pre\u2010eclampsia (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>20) and normal groups (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>20) was first screened by microarray analysis and validated by quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> analysis. The integrated bioinformatics analysis showed that miR\u201016 showed the highest number of connections in the mi<jats:styled-content style=\"fixed-case\">RNA GO<\/jats:styled-content> network and the mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> gene network. Moreover, over\u2010expressed miR\u201016 inhibited the proliferation and migration of decidua\u2010derived mesenchymal stem cells and induced cell\u2010cycle arrest by targeting cyclin\u00a0E1. Interestingly, over\u2010expression of miR\u201016 by decidua\u2010derived mesenchymal stem cells reduced the ability of human umbilical vein endothelial cells to form blood vessels and reduced the migration of trophoblast cells. Furthermore, decidua\u2010derived mesenchymal stem cell\u2010expressed endothelial growth factor <jats:styled-content style=\"fixed-case\">VEGF\u2010A<\/jats:styled-content> was involved in migration of trophoblast cells and human umbilical vein endothelial cells as well as tube and network formation. Importantly, the levels of cyclin\u00a0E1 and <jats:styled-content style=\"fixed-case\">VEGF\u2010A<\/jats:styled-content> were negatively correlated with the level of miR\u201016 expression in decidua\u2010derived mesenchymal stem cells from the patients with severe pre\u2010eclampsia. Together, these data suggest that the alteration of miR\u201016 expression in decidua\u2010derived mesenchymal stem cells may be involved in the development of pre\u2010eclampsia.<\/jats:p>","DOI":"10.1111\/febs.12037","type":"journal-article","created":{"date-parts":[[2012,10,19]],"date-time":"2012-10-19T18:05:09Z","timestamp":1350669909000},"page":"4510-4524","source":"Crossref","is-referenced-by-count":102,"title":["miR\u201016 inhibits the proliferation and angiogenesis\u2010regulating potential of mesenchymal stem cells in severe pre\u2010eclampsia"],"prefix":"10.1111","volume":"279","author":[{"given":"Yaping","family":"Wang","sequence":"first","affiliation":[{"name":"Immunology and Reproductive Biology Laboratory, Medical School &amp; State Key Laboratory of Pharmaceutical Biotechnology Nanjing University China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongye","family":"Fan","sequence":"additional","affiliation":[{"name":"Immunology and Reproductive 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