{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T06:01:54Z","timestamp":1785564114981,"version":"3.56.0"},"reference-count":21,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2013,8,23]],"date-time":"2013-08-23T00:00:00Z","timestamp":1377216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:sec><jats:label\/><jats:p>Resistance of <jats:italic><jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>eishmania<\/jats:italic> parasites to miltefosine, which is only available oral drug, is a great concern. We have analyzed global gene expression profiles of miltefosine\u2010unresponsive and miltefosine\u2010responsive <jats:italic><jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>eishmania\u00a0donovani<\/jats:italic> in order to understand the various metabolic processes involved in miltefosine drug resistance. The microarray data clearly indicated a role of oxidative metabolism in miltefosine resistance. Furthermore, fluorescence microscopy experiments suggested that miltefosine\u2010unresponsive <jats:italic><jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>.\u00a0donovani<\/jats:italic> resists the accumulation of reactive oxygen species and subsequent mitochondrial membrane damage leading to apoptotic death. In contrast, in miltefosine\u2010responsive <jats:italic><jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>.\u00a0donovani<\/jats:italic>, the accumulation of reactive oxygen species causes apoptotic death. Overall, this study provides fundamental insights into miltefosine resistance in <jats:italic><jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>.\u00a0donovani<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Database<\/jats:title><jats:p>The microarray data have been deposited in the <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>ene <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>xpression <jats:styled-content style=\"fixed-case\">O<\/jats:styled-content>mnibus database under the accession number <jats:styled-content style=\"fixed-case\">GSE<\/jats:styled-content>45496<\/jats:p><\/jats:sec>","DOI":"10.1111\/febs.12449","type":"journal-article","created":{"date-parts":[[2013,7,25]],"date-time":"2013-07-25T17:06:00Z","timestamp":1374771960000},"page":"4807-4815","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Miltefosine\u2010unresponsive 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