{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T17:30:22Z","timestamp":1769275822586,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,11,5]],"date-time":"2013-11-05T00:00:00Z","timestamp":1383609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:p>Phosphatase and tensin homologue deleted on chromosome 10 (<jats:styled-content style=\"fixed-case\">PTEN<\/jats:styled-content>) has been reported to play a role in the suppression of activated hepatic stellate cells (<jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s). Moreover, it has been demonstrated that hypermethylation of the <jats:styled-content style=\"fixed-case\">PTEN<\/jats:styled-content> promoter is responsible for the loss of <jats:styled-content style=\"fixed-case\">PTEN<\/jats:styled-content> expression during <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content> activation. Methylation is now established as a fundamental regulator of gene transcription. Micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s), which can control gene expression by binding to their target genes for degradation and\/or translational repression, were found to be involved in liver fibrosis. However, the mechanism responsible for mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010mediated epigenetic regulation in liver fibrosis still remained unclear. In the present study, curcumin treatment significantly resulted in the inhibition of cell proliferation and an increase in the apoptosis rate through the up\u2010regulation of <jats:styled-content style=\"fixed-case\">PTEN<\/jats:styled-content> associated with a decreased <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation level. Only <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methyltransferase 3b (<jats:styled-content style=\"fixed-case\">DNMT<\/jats:styled-content>3b) was reduced <jats:italic>in\u00a0vivo<\/jats:italic> and <jats:italic>in\u00a0vitro<\/jats:italic> after curcumin treatment. Further studies were performed aiming to confirm that the knockdown of <jats:styled-content style=\"fixed-case\">DNMT<\/jats:styled-content>3b enhanced the loss of <jats:styled-content style=\"fixed-case\">PTEN<\/jats:styled-content> methylation by curcumin. In addition, miR\u201029b was involved in the hypomethylation of <jats:styled-content style=\"fixed-case\">PTEN<\/jats:styled-content> by curcumin. MiR\u201029b not only was increased by curcumin in activated <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s, but also was confirmed to target <jats:styled-content style=\"fixed-case\">DNMT<\/jats:styled-content>3b by luciferase activity assays. Curcumin\u2010mediated <jats:styled-content style=\"fixed-case\">PTEN<\/jats:styled-content> up\u2010regulation, <jats:styled-content style=\"fixed-case\">DNMT<\/jats:styled-content>3b down\u2010regulation and <jats:styled-content style=\"fixed-case\">PTEN<\/jats:styled-content> hypomethylation were all attenuated by miR\u201029b inhibitor. Collectively, it is demonstrated that curcumin can up\u2010regulate miR\u201029b expression, resulting in <jats:styled-content style=\"fixed-case\">DNMT<\/jats:styled-content>3b down\u2010regulation in <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s and epigenetically\u2010regulated <jats:styled-content style=\"fixed-case\">PTEN<\/jats:styled-content> involved in the suppression of activated <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s. These results indicate that mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010mediated epigenetic regulation may be a novel mechanism suppressing liver fibrosis.<\/jats:p>","DOI":"10.1111\/febs.12574","type":"journal-article","created":{"date-parts":[[2013,10,21]],"date-time":"2013-10-21T08:16:36Z","timestamp":1382343396000},"page":"88-103","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":89,"title":["Curcumin up\u2010regulates phosphatase and tensin homologue deleted on chromosome 10 through micro<scp>RNA<\/scp>\u2010mediated control of <scp>DNA<\/scp> methylation\u00a0\u2013\u00a0a novel mechanism suppressing liver fibrosis"],"prefix":"10.1111","volume":"281","author":[{"given":"Jianjian","family":"Zheng","sequence":"first","affiliation":[{"name":"Wenzhou Key Laboratory of Surgery The First Affiliated Hospital of Wenzhou Medical University China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cunzao","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Organ Transplantation The First Affiliated Hospital of Wenzhou Medical University China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuo","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases The First Affiliated Hospital of Wenzhou Medical University China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Guo","sequence":"additional","affiliation":[{"name":"Institute of Organ Transplantation The First Affiliated Hospital of Wenzhou Medical University China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine The First Affiliated Hospital of Wenzhou Medical University China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peihong","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases The First Affiliated Hospital of Wenzhou Medical University China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongqiu","family":"Lu","sequence":"additional","affiliation":[{"name":"Emergency Department The First Affiliated Hospital of Wenzhou Medical University China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shenmeng","family":"Gao","sequence":"additional","affiliation":[{"name":"Laboratory of Internal Medicine The First Affiliated Hospital of Wenzhou Medical University China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Liao","sequence":"additional","affiliation":[{"name":"Hepatobiliary Surgery The First Affiliated Hospital of Wenzhou Medical University China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bicheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Wenzhou Key Laboratory of Surgery The First Affiliated Hospital of Wenzhou Medical University China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fujun","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases The First Affiliated Hospital of Wenzhou Medical University China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,11,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2012.00069"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024568"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2001-17558"},{"key":"e_1_2_7_5_1","first-page":"1069","article-title":"The hepatic perisinusoidal stellate cell","volume":"12","author":"Kawada N","year":"1997","journal-title":"Histol Histopathol"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2007.1769"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2007.08.016"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.40.174"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11418-009-0347-3"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-3648"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00542.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2009.09.013"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-006-9142-7"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2012.06.022"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.74.010904.153721"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2290"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2012.00049"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.11.056"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.07.041"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707628104"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-07-170589"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2010.11.035"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2011.01.009"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-010-1304-2"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00474.2002"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20040928"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0707542"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00220.2009"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M809787200"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.2764"},{"key":"e_1_2_7_32_1","first-page":"99","article-title":"Isolation and culture of hepatic stellate cells","volume":"117","author":"Weiskirchen R","year":"2005","journal-title":"Methods Mol Med"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-12-156"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"}],"updated-by":[{"DOI":"10.1111\/febs.16988","type":"expression_of_concern","label":"Expression of concern","source":"publisher","updated":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T00:00:00Z","timestamp":1700524800000}}],"container-title":["The FEBS Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ffebs.12574","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.12574","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T12:50:19Z","timestamp":1696251019000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/febs.12574"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,5]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1111\/febs.12574"],"URL":"https:\/\/doi.org\/10.1111\/febs.12574","archive":["Portico"],"relation":{},"ISSN":["1742-464X","1742-4658"],"issn-type":[{"value":"1742-464X","type":"print"},{"value":"1742-4658","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,5]]},"assertion":[{"value":"2013-07-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-10-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-11-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}