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When combined with polygodial, nagilactone\u00a0<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>, and <jats:italic>n<\/jats:italic>\u2010dodecanol, anethole has been shown to possess significant synergistic antifungal activity against a budding yeast, <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>accharomyces<\/jats:italic>\u00a0<jats:italic>cerevisiae<\/jats:italic>, and a human opportunistic pathogenic yeast, <jats:italic><jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>andida\u00a0albicans<\/jats:italic>. However, the antifungal mechanism of anethole has not been completely determined. We found that anethole stimulated cell death of a human opportunistic pathogenic fungus, <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>spergillus\u00a0fumigatus<\/jats:italic>, in addition to <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>.\u00a0cerevisiae<\/jats:italic>. The anethole\u2010induced cell death was accompanied by reactive oxygen species production, metacaspase activation, and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> fragmentation. Several mutants of <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>.\u00a0cerevisiae<\/jats:italic>, in which genes related to the apoptosis\u2010initiating execution signals from mitochondria were deleted, were resistant to anethole. These results suggest that anethole\u2010induced cell death could be explained by oxidative stress\u2010dependent apoptosis via typical mitochondrial death cascades in fungi, including <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>.\u00a0fumigatus<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>.\u00a0cerevisiae<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/febs.12706","type":"journal-article","created":{"date-parts":[[2014,1,2]],"date-time":"2014-01-02T17:37:55Z","timestamp":1388684275000},"page":"1304-1313","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Anethole induces apoptotic cell death accompanied by reactive oxygen species production and <scp>DNA<\/scp> fragmentation in <i><scp>A<\/scp>spergillus\u00a0fumigatus<\/i> and 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