{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T22:24:55Z","timestamp":1771712695223,"version":"3.50.1"},"reference-count":77,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,2,19]],"date-time":"2014-02-19T00:00:00Z","timestamp":1392768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2014,4]]},"abstract":"Carbonic anhydrases (CA<\/jats:styled-content>s) are metalloenzymes catalyzing the reversible hydration of carbon dioxide to bicarbonate (hydrogen carbonate) and protons. CA<\/jats:styled-content>s have been identified in archaea, bacteria and eukaryotes and can be classified into five groups (\u03b1, \u03b2, \u03b3, \u03b4, \u03b6) that are unrelated in sequence and structure. The fungal \u03b2\u2010class has only recently attracted attention. In the present study, we investigated the structure and function of the plant\u2010like \u03b2\u2010CA<\/jats:styled-content> proteins CAS<\/jats:styled-content>1 and CAS<\/jats:styled-content>2 from the filamentous ascomycete Sordaria\u00a0macrospora<\/jats:italic>. We demonstrated that both proteins can substitute for the Saccharomyces\u00a0cerevisiae<\/jats:italic> \u03b2\u2010CA<\/jats:styled-content> Nce103 and exhibit an in\u00a0vitro <\/jats:italic>CO<\/jats:styled-content>2<\/jats:sub> hydration activity (k<\/jats:italic>cat<\/jats:sub>\/K<\/jats:italic>m<\/jats:sub> of CAS<\/jats:styled-content>1:\u00a01.30\u00a0\u00d7\u00a0106<\/jats:sup>\u00a0m<\/jats:sc>\u22121<\/jats:sup>\u00b7s\u22121<\/jats:sup>; CAS<\/jats:styled-content>2:\u00a01.21\u00a0\u00d7\u00a0106\u00a0<\/jats:sup>m<\/jats:sc>\u22121<\/jats:sup>\u00b7s\u22121<\/jats:sup>). To further investigate the structural properties of CAS<\/jats:styled-content>1 and CAS<\/jats:styled-content>2, we determined their crystal structures to a resolution of 2.7\u00a0\u00c5 and 1.8\u00a0\u00c5, respectively. The oligomeric state of both proteins is tetrameric. With the exception of the active site composition, no further major differences have been found. In both enzymes, the Zn2<\/jats:sup>+<\/jats:sup>\u00a0\u2010ion is tetrahedrally coordinated; in CAS<\/jats:styled-content>1 by Cys45, His101 and Cys104 and a water molecule and in CAS<\/jats:styled-content>2 by the side chains of four residues (Cys56, His112, Cys115 and Asp58). Both CA<\/jats:styled-content>s are only weakly inhibited by anions, making them good candidates for industrial applications.<\/jats:p><\/jats:sec>Structured digital abstract<\/jats:title>\nCAS1<\/jats:ext-link>\u00a0and\u00a0CAS2<\/jats:ext-link>\u00a0bind<\/jats:ext-link>\u00a0by\u00a0x-ray crystallography<\/jats:ext-link>\u00a0(View interaction<\/jats:ext-link>)<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>Database<\/jats:title>Structural data have been deposited in the Protein Data Bank database under accession numbers 4O1J<\/jats:ext-link> for CAS1 and 4O1K<\/jats:ext-link> for CAS2.<\/jats:p><\/jats:sec>","DOI":"10.1111\/febs.12738","type":"journal-article","created":{"date-parts":[[2014,2,7]],"date-time":"2014-02-07T14:43:07Z","timestamp":1391784187000},"page":"1759-1772","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Crystal structures of two tetrameric \u03b2\u2010carbonic anhydrases from the filamentous ascomycete Sordaria\u00a0macrospora<\/i>"],"prefix":"10.1111","volume":"281","author":[{"given":"Ronny","family":"Lehneck","sequence":"first","affiliation":[{"name":"Institute of Microbiology and Genetics Department of Genetics of Eukaryotic Microorganisms Georg\u2010August\u2010University G\u00f6ttingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piotr","family":"Neumann","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Genetics Department of Molecular Structural Biology Georg\u2010August\u2010University G\u00f6ttingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Vullo","sequence":"additional","affiliation":[{"name":"Dipartimento di Chimica Ugo Schiff Universit\u00e0 degli Studi di Firenze Sesto Fiorentino Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Skander","family":"Elleuche","sequence":"additional","affiliation":[{"name":"Institute of Technical Microbiology Hamburg University of Technology Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudiu T.","family":"Supuran","sequence":"additional","affiliation":[{"name":"Dipartimento di Chimica Ugo Schiff Universit\u00e0 degli Studi di Firenze Sesto Fiorentino Florence Italy"},{"name":"Neurofarba Department Section of Pharmaceutical and Nutriceutical Sciences Universit\u00e0 degli Studi di Firenze Sesto Fiorentino Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf","family":"Ficner","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Genetics Department of Molecular Structural Biology Georg\u2010August\u2010University G\u00f6ttingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"P\u00f6ggeler","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Genetics Department of Genetics of Eukaryotic Microorganisms Georg\u2010August\u2010University G\u00f6ttingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,2,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000047375"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.09.047"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.10.040"},{"key":"e_1_2_7_5_1","first-page":"15","article-title":"Carbon dioxide\u2010sensing in organisms and its implications for human disease","volume":"1","author":"Cummins EP","year":"2013","journal-title":"Cell Mol Life 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