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Substantial evidence indicates that the membrane\u2010mediated aggregation and subsequent deposition of hI<jats:styled-content style=\"fixed-case\">APP<\/jats:styled-content> are linked to dysfunction and death of pancreatic \u03b2\u2010cells, but the molecular processes of hI<jats:styled-content style=\"fixed-case\">APP<\/jats:styled-content> deposition are poorly understood. In this study, we examined the membrane\u2010mediated aggregation and deposition of hI<jats:styled-content style=\"fixed-case\">APP<\/jats:styled-content> at supported planar lipid bilayers with and without raft components (i.e. cholesterol and sphingomyelin) to provide insight into hI<jats:styled-content style=\"fixed-case\">APP<\/jats:styled-content>\u2010induced membrane dysfunction. The adsorption of hI<jats:styled-content style=\"fixed-case\">APP<\/jats:styled-content> onto the bilayers was studied using a quartz crystal microbalance with dissipation monitoring, which showed enhanced accumulation of the peptide onto the bilayer containing raft components. Microscope observations demonstrated the growth of the aggregates formed from the membrane\u2010adsorbed hI<jats:styled-content style=\"fixed-case\">APP<\/jats:styled-content>. The examination of the membrane interfaces revealed that hI<jats:styled-content style=\"fixed-case\">APP<\/jats:styled-content> aggregates retained the ability to associate with the membranes during the aggregation process, resulting in insertion of the aggregates into the bilayers. We also report the inhibitory effect of insulin on the hI<jats:styled-content style=\"fixed-case\">APP<\/jats:styled-content> deposition. These findings demonstrate the aggregation of hI<jats:styled-content style=\"fixed-case\">APP<\/jats:styled-content> at the membrane interfaces leading to amyloid deposits associated with the membrane and suggest a role for insulin in hI<jats:styled-content style=\"fixed-case\">APP<\/jats:styled-content> deposition. A presumed mechanism regulating hI<jats:styled-content style=\"fixed-case\">APP<\/jats:styled-content> deposition at the membrane interfaces is discussed.<\/jats:p>","DOI":"10.1111\/febs.12807","type":"journal-article","created":{"date-parts":[[2014,4,7]],"date-time":"2014-04-07T05:12:44Z","timestamp":1396847564000},"page":"2597-2612","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Amyloid aggregation and deposition of human islet amyloid polypeptide at membrane interfaces"],"prefix":"10.1111","volume":"281","author":[{"given":"Kenji","family":"Sasahara","sequence":"first","affiliation":[{"name":"Department of Microbiology and Infectious Diseases Graduate School of Medicine Kobe University Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenichi","family":"Morigaki","sequence":"additional","affiliation":[{"name":"Research Center for Environmental Genomics Kobe University 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