{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:18:47Z","timestamp":1791418727369,"version":"4.3.3"},"reference-count":53,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2014,7,21]],"date-time":"2014-07-21T00:00:00Z","timestamp":1405900800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:p>Poly(<jats:styled-content style=\"fixed-case\">ADP<\/jats:styled-content>\u2010ribose) (<jats:styled-content style=\"fixed-case\">PAR<\/jats:styled-content>) is a complex and reversible post\u2010translational modification that controls protein function and localization through covalent modification of, or noncovalent binding to target proteins. Previously, we and others characterized the noncovalent, high\u2010affinity binding of the key nucleotide excision repair (<jats:styled-content style=\"fixed-case\">NER<\/jats:styled-content>) protein <jats:styled-content style=\"fixed-case\">XPA<\/jats:styled-content> to <jats:styled-content style=\"fixed-case\">PAR<\/jats:styled-content>. In the present study, we address the functional relevance of this interaction. First, we confirm that pharmacological inhibition of cellular poly(<jats:styled-content style=\"fixed-case\">ADP<\/jats:styled-content>\u2010ribosyl)ation (<jats:styled-content style=\"fixed-case\">PAR<\/jats:styled-content>ylation) impairs <jats:styled-content style=\"fixed-case\">NER<\/jats:styled-content> efficacy. Second, we demonstrate that the <jats:styled-content style=\"fixed-case\">XPA<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">PAR<\/jats:styled-content> interaction is mediated by specific basic amino acids within a highly conserved <jats:styled-content style=\"fixed-case\">PAR<\/jats:styled-content>\u2010binding motif, which overlaps the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage\u2010binding protein 2 (<jats:styled-content style=\"fixed-case\">DDB<\/jats:styled-content>2) and transcription factor <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> H (<jats:styled-content style=\"fixed-case\">TFIIH<\/jats:styled-content>) interaction domains of <jats:styled-content style=\"fixed-case\">XPA<\/jats:styled-content>. Third, biochemical studies reveal a mutual regulation of <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">XPA<\/jats:styled-content> functions showing that, on the one hand, the <jats:styled-content style=\"fixed-case\">XPA<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">PAR<\/jats:styled-content> interaction lowers the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> binding affinity of <jats:styled-content style=\"fixed-case\">XPA<\/jats:styled-content>, whereas, on the other hand, <jats:styled-content style=\"fixed-case\">XPA<\/jats:styled-content> itself strongly stimulates <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>1 enzymatic activity. Fourth, microirradiation experiments in U2<jats:styled-content style=\"fixed-case\">OS<\/jats:styled-content> cells demonstrate that <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content> inhibition alters the recruitment properties of <jats:styled-content style=\"fixed-case\">XPA<\/jats:styled-content>\u2010green fluorescent protein to sites of laser\u2010induced <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage. In conclusion, our results reveal that <jats:styled-content style=\"fixed-case\">XPA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>1 regulate each other in a reciprocal and <jats:styled-content style=\"fixed-case\">PAR<\/jats:styled-content>\u2010dependent manner, potentially acting as a fine\u2010tuning mechanism for the spatio\u2010temporal regulation of the two factors during <jats:styled-content style=\"fixed-case\">NER<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/febs.12885","type":"journal-article","created":{"date-parts":[[2014,6,23]],"date-time":"2014-06-23T06:02:10Z","timestamp":1403503330000},"page":"3625-3641","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["Poly(<scp>ADP<\/scp>\u2010ribose)\u2010mediated interplay of <scp>XPA<\/scp> and <scp>PARP<\/scp>1 leads to reciprocal regulation of protein function"],"prefix":"10.1111","volume":"281","author":[{"given":"Jan M. F.","family":"Fischer","sequence":"first","affiliation":[{"name":"Molecular Toxicology Group Department of Biology University of Konstanz Germany"},{"name":"Konstanz Research School Chemical Biology University of Konstanz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Popp","sequence":"additional","affiliation":[{"name":"Molecular Toxicology Group Department of Biology University of Konstanz Germany"},{"name":"Konstanz Research School Chemical Biology University of Konstanz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Gebhard","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Albstadt\u2010Sigmaringen University of Applied Sciences Sigmaringen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Veith","sequence":"additional","affiliation":[{"name":"Molecular Toxicology Group Department of Biology University of Konstanz Germany"},{"name":"Research Training Group 1331 University of Konstanz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur","family":"Fischbach","sequence":"additional","affiliation":[{"name":"Molecular Toxicology Group Department of Biology University of Konstanz Germany"},{"name":"Konstanz Research School Chemical Biology University of Konstanz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sascha","family":"Beneke","sequence":"additional","affiliation":[{"name":"Molecular Toxicology Group Department of Biology University of Konstanz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfred","family":"Leitenstorfer","sequence":"additional","affiliation":[{"name":"Department of Physics and Center for Applied Photonics University of Konstanz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Bergemann","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Albstadt\u2010Sigmaringen University of Applied Sciences Sigmaringen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Scheffner","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biochemistry Department of Biology University of Konstanz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Ferrando\u2010May","sequence":"additional","affiliation":[{"name":"Bioimaging Center University of Konstanz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aswin","family":"Mangerich","sequence":"additional","affiliation":[{"name":"Molecular Toxicology Group Department of Biology University of Konstanz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"B\u00fcrkle","sequence":"additional","affiliation":[{"name":"Molecular Toxicology Group Department of Biology University of Konstanz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/321653"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2009.12.003"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2013.02.001"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb400170b"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3164"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050889"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2012.12.005"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3993"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2013.08.026"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3240"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01636"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1209507110"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2008.01.001"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2013.03.002"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201112132"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201106074"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.393504"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2012.06.004"},{"key":"e_1_2_8_20_1","first-page":"4053","article-title":"3\u2010Aminobenzamide can act as a cocarcinogen for ultraviolet light\u2010induced carcinogenesis in mouse skin","volume":"52","author":"Epstein JH","year":"1992","journal-title":"Cancer Res"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn964"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-09599-8_4"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006520200"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm944"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb300363g"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb300317y"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2011.07.006"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-10-46"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.19.11839"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp932"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.200710019"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm933"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt025"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3376"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn771"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.11.2779"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/1400"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.37.22991"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.9.4896"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks486"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2603"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2013.00290"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00121-09"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2011.03.004"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.07.006"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2004.10.007"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-009-9472-z"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1568-7864(02)00053-8"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.255869"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100492"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2010.04.003"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1993.1004"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.13.3554"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.5520310305"}],"container-title":["The FEBS Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ffebs.12885","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.12885","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/febs.12885","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.12885","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T07:50:23Z","timestamp":1696319423000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/febs.12885"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,21]]},"references-count":53,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1111\/febs.12885"],"URL":"https:\/\/doi.org\/10.1111\/febs.12885","archive":["Portico"],"relation":{},"ISSN":["1742-464X","1742-4658"],"issn-type":[{"value":"1742-464X","type":"print"},{"value":"1742-4658","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,21]]},"assertion":[{"value":"2014-04-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-06-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-07-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}