{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T02:57:19Z","timestamp":1773111439626,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2014,7,31]],"date-time":"2014-07-31T00:00:00Z","timestamp":1406764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology , Ministry of Science and Technology","doi-asserted-by":"publisher","award":["BT\/PR1822\/AGR\/36\/678\/2011"],"award-info":[{"award-number":["BT\/PR1822\/AGR\/36\/678\/2011"]}],"id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:p>MicroRNAs control cellular processes by regulating expression of their target genes. Here we report that neuro\u2010epithelial transforming gene 1 (NET1) is a target of tumor suppressor microRNA 22 (miR\u201022). miR\u201022 is downregulated in peripheral blood mononuclear cells derived from chronic myeloid leukemia (<jats:styled-content style=\"fixed-case\">CML<\/jats:styled-content>) patients and in CML cell line K562. NET1 was identified as one of the targets of miR\u201022 using both <jats:italic>in\u00a0vitro<\/jats:italic> and <jats:italic>in\u00a0vivo<\/jats:italic> experiments. Either mutations or naturally occurring single\u2010nucleotide polymorphisms in NET1 3\u2032\u2010UTR that map at the miR\u201022 binding site were found to affect binding of miR\u201022 to NET1 mRNA. Over expression of NET1 in K562 cells resulted in increased proliferation. However decreased proliferation and alteration in cell cycle were observed on either overexpression of miR\u201022 or knockdown of NET1 expression respectively. We also found that overexpression of miR\u201022 or NET1 knockdown inhibits actin fiber formation, probably by downregulation of NET1 as NET1 knockdown also resulted in depletion of actin fiber formation. We suggest that the oncogenic properties of CML cells are probably due to deregulated expression of NET1 as a result of altered expression of miR\u201022.<\/jats:p>","DOI":"10.1111\/febs.12926","type":"journal-article","created":{"date-parts":[[2014,7,9]],"date-time":"2014-07-09T11:47:41Z","timestamp":1404906461000},"page":"3904-3919","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["miR\u201022 regulates expression of oncogenic neuro\u2010epithelial transforming gene 1, <scp>NET<\/scp>1"],"prefix":"10.1111","volume":"281","author":[{"given":"Hafiz M.","family":"Ahmad","sequence":"first","affiliation":[{"name":"School of Life Sciences Jawaharlal Nehru University New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamchui","family":"Muiwo","sequence":"additional","affiliation":[{"name":"School of Life Sciences Jawaharlal Nehru University New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suganthi S.","family":"Ramachandran","sequence":"additional","affiliation":[{"name":"Department of Pharmacology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priyatama","family":"Pandey","sequence":"additional","affiliation":[{"name":"School of Computational and Integrative Sciences Jawaharlal Nehru University New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yogendra K.","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Pharmacology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lalit","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Institute Rotary Cancer Hospital All India Institute of Medical Science New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ritu","family":"Kulshreshtha","sequence":"additional","affiliation":[{"name":"Department of Biochemical Engineering and Biotechnology Indian Institute of Technology New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alok","family":"Bhattacharya","sequence":"additional","affiliation":[{"name":"School of Life Sciences Jawaharlal Nehru University New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7,31]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.04.003"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10888"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2708"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2012.02346.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-01-136218"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-08-0167"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-09-045104"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2008.04.018"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2013.02.002"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-11-288"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.03.030"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.112.300682"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-013-0542-7"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020291"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2013.2300"},{"key":"e_1_2_8_17_1","first-page":"1835","article-title":"Tiam1, negatively regulated by miR\u201022, miR\u2010183 and miR\u201031, is involved in migration, invasion and viability of ovarian cancer cells","volume":"27","author":"Li J","year":"2012","journal-title":"Oncol Rep"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-012-1194-2"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.0551"},{"key":"e_1_2_8_20_1","first-page":"828","article-title":"miR\u2010324\u20105p inhibits proliferation of glioma by target regulation of GLI1","volume":"18","author":"Xu HS","year":"2014","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050895"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2010.07594.x"},{"key":"e_1_2_8_23_1","volume-title":"Quantitative Human Physiology: An Introduction","author":"Feher JJ","year":"2012"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.5.3.2393"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms14048179"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.14.4075"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp683"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2008.09.001"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401221"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.060613"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M009534200"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V54.3.713.713"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027774"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-012-1123-1"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-11-50"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00175-13"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2011.457"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062589"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2013.06.003"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.67"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn851"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-6-390"}],"container-title":["The FEBS Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ffebs.12926","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.12926","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T05:19:04Z","timestamp":1696310344000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/febs.12926"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,31]]},"references-count":42,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2014,9]]}},"alternative-id":["10.1111\/febs.12926"],"URL":"https:\/\/doi.org\/10.1111\/febs.12926","archive":["Portico"],"relation":{},"ISSN":["1742-464X","1742-4658"],"issn-type":[{"value":"1742-464X","type":"print"},{"value":"1742-4658","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,31]]},"assertion":[{"value":"2014-01-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-07-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-07-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}