{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T19:02:25Z","timestamp":1761418945946,"version":"3.37.3"},"reference-count":40,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2014,9,19]],"date-time":"2014-09-19T00:00:00Z","timestamp":1411084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2012R1A22007113"],"award-info":[{"award-number":["2012R1A22007113"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:p>The Edc3 protein is an enhancer of m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> decapping, and acts as a scaffold protein for the m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> granules that are known as processing bodies in yeast. In the pathogenic yeast <jats:italic>Candida\u00a0albicans<\/jats:italic>, various stresses, such as glucose depletion, oxidative stress, and filamentation defects, induce the accumulation of processing bodies. Here, we report that the <jats:italic>edc3<\/jats:italic>\/<jats:italic>edc3<\/jats:italic> deletion strain showed increased resistance to various stresses, including hydrogen peroxide, acetic acid, and high temperature. Oxidative stress is known to induce the intracellular accumulation of reactive oxygen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>) and apoptotic cell death in <jats:italic>C.\u00a0albicans<\/jats:italic>. We found that the <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> level was lower in <jats:italic>edc3\/edc3<\/jats:italic> cells than in wild\u2010type cells following oxidative stress. We also observed that expression of the metacaspase gene <jats:italic>Ca<jats:styled-content style=\"fixed-case\">MCA<\/jats:styled-content>1<\/jats:italic> was decreased in <jats:italic>edc3<\/jats:italic>\/<jats:italic>edc3<\/jats:italic> cells. Overexpression of <jats:italic>Ca<jats:styled-content style=\"fixed-case\">MCA<\/jats:styled-content>1<\/jats:italic> suppressed the decreased accumulation of <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> and the increased resistance to hydrogen peroxide in <jats:italic>edc3<\/jats:italic>\/<jats:italic>edc3<\/jats:italic> cells. The catalase Cat1 and the superoxide dismutase Sod1 were upregulated in <jats:italic>edc3<\/jats:italic>\/<jats:italic>edc3<\/jats:italic> cells as compared with wild\u2010type cells. On the basis of these findings, we suggest that <jats:italic><jats:styled-content style=\"fixed-case\">EDC<\/jats:styled-content>3<\/jats:italic> plays a critical role in the expression of <jats:italic>Ca<jats:styled-content style=\"fixed-case\">MCA<\/jats:styled-content>1<\/jats:italic> and the oxidative stress response in <jats:italic>C.\u00a0albicans<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/febs.13022","type":"journal-article","created":{"date-parts":[[2014,8,27]],"date-time":"2014-08-27T05:35:54Z","timestamp":1409117754000},"page":"4841-4851","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Roles of Edc3 in the oxidative stress response and <i>Ca<scp>MCA<\/scp>1\u2010<\/i>encoded metacaspase expression in <i>Candida\u00a0albicans<\/i>"],"prefix":"10.1111","volume":"281","author":[{"given":"Jong\u2010Hwan","family":"Jung","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular 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