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Estrogen receptor (ER<\/jats:styled-content>) \u03b1, in contrast to ER<\/jats:styled-content>\u03b2, has been implicated in the induction of PR<\/jats:styled-content>s. The simultaneous activation of ER<\/jats:styled-content>\u03b1 and ER<\/jats:styled-content>\u03b2, although not increasing the number of PR<\/jats:styled-content>\u2010immunoreactive neurons in the VMN<\/jats:styled-content>vl, facilitates lordosis, which suggests that ER<\/jats:styled-content>\u03b2 and\/or the ER<\/jats:styled-content>\u03b1\u2013ER<\/jats:styled-content>\u03b2 interaction might play a role in PR<\/jats:styled-content> dynamics and\/or PR<\/jats:styled-content> expression by individual neurons. To address this question, we used western blot and immunohistochemical studies to determine the amounts and subcellular distributions of both PR<\/jats:styled-content> isoforms in VMN<\/jats:styled-content>vl neurons of ovariectomized rats injected with estradiol benzoate or with specific agonists of ER<\/jats:styled-content>\u03b1 and ER<\/jats:styled-content>\u03b2, alone or in association. The present data show that ER<\/jats:styled-content>\u03b1 activation does not change PR<\/jats:styled-content> expression in individual neurons, but increases the number of PR<\/jats:styled-content>s in the VMN<\/jats:styled-content>vl, because it increases the number of neurons expressing PR<\/jats:styled-content>s. Conversely, ER<\/jats:styled-content>\u03b2 activation does not change the total number of PR<\/jats:styled-content>s in the VMN<\/jats:styled-content>vl, but increases the labeling intensity of the perikaryal cytoplasm, which suggests that it promotes the transport of PR<\/jats:styled-content>s from neurites into cell bodies. In addition, the simultaneous activation of ER<\/jats:styled-content>\u03b1 and ER<\/jats:styled-content>\u03b2 increases the expression of PR<\/jats:styled-content>s by individual neurons and, consequently, increases the total number of PR<\/jats:styled-content>s in the VMN<\/jats:styled-content>vl. Our findings reveal that individual and simultaneous activation of ER<\/jats:styled-content>\u03b1 and ER<\/jats:styled-content>\u03b2 have different effects on the levels and subcellular location of PR<\/jats:styled-content>s in VMN<\/jats:styled-content>vl neurons.<\/jats:p>","DOI":"10.1111\/febs.13207","type":"journal-article","created":{"date-parts":[[2015,1,22]],"date-time":"2015-01-22T17:09:08Z","timestamp":1421946548000},"page":"1126-1136","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Estrogen receptors \u03b1 and \u03b2 have different roles in the induction and trafficking of progesterone receptors in hypothalamic ventromedial neurons"],"prefix":"10.1111","volume":"282","author":[{"given":"Susana I.","family":"S\u00e1","sequence":"first","affiliation":[{"name":"Department of Anatomy Faculty of Medicine University of Porto Portugal"},{"name":"Center of Experimental Morphology (CME) Faculty of Medicine University of Porto Portugal"},{"name":"Center for Health Technology and Services Research (CINTESIS) Faculty of Medicine University of Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno M.","family":"Fonseca","sequence":"additional","affiliation":[{"name":"UCIBIO@REQUIMTE Laboratory of Biochemistry Department of Biological Sciences Faculty of Pharmacy University of Porto Portugal"},{"name":"Biology of Inflammation and Reproduction Institute for Molecular and Cell Biology Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nat\u00e9rcia","family":"Teixeira","sequence":"additional","affiliation":[{"name":"UCIBIO@REQUIMTE Laboratory of Biochemistry Department of Biological Sciences Faculty of Pharmacy University of Porto Portugal"},{"name":"Biology of Inflammation and Reproduction Institute for Molecular and Cell Biology Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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