{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T05:57:20Z","timestamp":1787810240214,"version":"build-2784847793"},"reference-count":25,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2015,2,11]],"date-time":"2015-02-11T00:00:00Z","timestamp":1423612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology of Japan","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec><jats:label\/><jats:p>The release of <jats:styled-content style=\"fixed-case\">SO<\/jats:styled-content><jats:sub>2<\/jats:sub> from petroleum products derived from crude oil, which contains sulfur compounds such as dibenzothiophene (<jats:styled-content style=\"fixed-case\">DBT<\/jats:styled-content>), leads to air pollution. The \u20184S\u2019 metabolic pathway catalyzes the sequential conversion of <jats:styled-content style=\"fixed-case\">DBT<\/jats:styled-content> to 2\u2010hydroxybiphenyl via three enzymes encoded by the <jats:italic>dsz<\/jats:italic> operon in several bacterial species. DszC (<jats:styled-content style=\"fixed-case\">DBT<\/jats:styled-content> monooxygenase), from <jats:italic>Rhodococcus\u00a0erythropolis<\/jats:italic> D\u20101 is involved in the first two steps of the \u20184S\u2019 pathway. Here, we determined the first crystal structure of <jats:styled-content style=\"fixed-case\">FMN<\/jats:styled-content>\u2010bound DszC, and found that two distinct conformations occur in the loop region (residues 131\u2013142) adjacent to the active site. On the basis of the DszC\u2013<jats:styled-content style=\"fixed-case\">FMN<\/jats:styled-content> structure and the previously reported apo structures of DszC homologs, the binding site for <jats:styled-content style=\"fixed-case\">DBT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">DBT<\/jats:styled-content> sulfoxide is proposed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Database<\/jats:title><jats:p>The atomic coordinates and structure factors for apo\u2010DszC (PDB code: <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.rcsb.org\/pdb\/search\/structidSearch.do?structureId=3X0X\">3X0X<\/jats:ext-link>) and DszC\u2010<jats:styled-content style=\"fixed-case\">FMN<\/jats:styled-content> (PDB code: <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.rcsb.org\/pdb\/search\/structidSearch.do?structureId=3X0Y\">3X0Y<\/jats:ext-link>) have been deposited in the Protein Data Bank (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.rcsb.org\">http:\/\/www.rcsb.org<\/jats:ext-link>).<\/jats:p><\/jats:sec>","DOI":"10.1111\/febs.13216","type":"journal-article","created":{"date-parts":[[2015,1,28]],"date-time":"2015-01-28T07:45:14Z","timestamp":1422431114000},"page":"3126-3135","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Crystal structures of apo\u2010DszC and FMN\u2010bound DszC from <i>Rhodococcus\u00a0erythropolis<\/i> D\u20101"],"prefix":"10.1111","volume":"282","author":[{"given":"Li\u2010Jun","family":"Guan","sequence":"first","affiliation":[{"name":"Department of Applied Biological Chemistry Graduate School of Agricultural and Life Sciences University of Tokyo Bunkyo\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Woo Cheol","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Applied 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