{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:53:14Z","timestamp":1790841194740,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,3,2]],"date-time":"2015-03-02T00:00:00Z","timestamp":1425254400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:sec><jats:label\/><jats:p>As in vertebrates, the insect steroid hormones, especially 20\u2010hydroxyecdysone (20E), initiate and regulate sexual behavior by acting on the central nervous system. This 20E action is, in part, triggered by transcriptional events mediated through the binding of 20E to a heterodimer comprising the ecdysone receptor (EcR) and ultraspiracle (<jats:styled-content style=\"fixed-case\">USP<\/jats:styled-content>). However, to date, our knowledge about this genomic steroid pathway remains incomplete. In moths, males detect female sex pheromones, eliciting stereotyped sexual behavior. In <jats:italic>Agrotis ipsilon<\/jats:italic> males, the behavioral response and the neuronal sensitivity to sex pheromone in the olfactory center, the antennal lobe (<jats:styled-content style=\"fixed-case\">AL<\/jats:styled-content>), increase with age. We recently showed that 20E controlled this age\u2010dependent olfactory plasticity via the activation of an EcR\/<jats:styled-content style=\"fixed-case\">USP<\/jats:styled-content>\u2010dependent pathway in the <jats:styled-content style=\"fixed-case\">AL<\/jats:styled-content>. Here, we cloned the gene encoding <jats:italic>A.\u00a0ipsilon<\/jats:italic> synaptotagmin I (<jats:italic>Ai<\/jats:italic>sytI), a presynaptic vesicle protein known to act as a calcium sensor in neurotransmitter release. <jats:italic>AisytI<\/jats:italic> was expressed in the <jats:styled-content style=\"fixed-case\">AL<\/jats:styled-content>, where its amount increased with age, whereas its knockdown inhibited the sex pheromone\u2010oriented flight of males. 20E administration to males induced <jats:styled-content style=\"fixed-case\">AL <\/jats:styled-content><jats:italic>AisytI<\/jats:italic> expression in a dose\u2010dependent and time\u2010dependent manner. Moreover, <jats:italic>A.\u00a0ipsilon EcR<\/jats:italic> silencing caused decreases in <jats:styled-content style=\"fixed-case\">AL <\/jats:styled-content><jats:italic>AisytI<\/jats:italic> expression and the behavioral response to sex pheromone. Our results show that the synaptotagmin I gene is a target gene for the genomic steroid signaling that controls the expression of insect sexual behavior by acting on central sex pheromone processing. This study thus represents a significant advance in our understanding of the steroid actions that influence neural functions, and thereby behavioral plasticity, in various organisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Database<\/jats:title><jats:p>The nucleotide sequence of Agrotis ipsilon synaptotagmin I is available in the DDBJ\/EMBL\/GenBank databases under the accession number <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.ncbi.nlm.nih.gov\/protein\/KJ863735\">KJ863735<\/jats:ext-link>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/febs.13231","type":"journal-article","created":{"date-parts":[[2015,2,13]],"date-time":"2015-02-13T13:38:10Z","timestamp":1423834690000},"page":"1432-1444","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Synaptotagmin I, a molecular target for steroid hormone signaling controlling the maturation of sexual behavior in an 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