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To generate a mouse model that allows such visualization via\n                    <jats:italic>in\u00a0vivo<\/jats:italic>\n                    fluorescence imaging, we crossed Prox1\u2013\n                    <jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>\n                    mice with Flk1::myr\u2013mCherry mice to generate Prox1\u2013\n                    <jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>\n                    \/Flk1::myr\u2013mCherry mice, in which lymphatic vessels emit green fluorescence and blood vessels emit red fluorescence. Corneal neovascularization was induced in these mice using three injury models: implantation of a vascular endothelial growth factor (\n                    <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>\n                    ) pellet, implantation of a basic fibroblast growth factor (bFGF) pellet, and alkali burn injury. Vessel growth was observed\n                    <jats:italic>in\u00a0vivo<\/jats:italic>\n                    by stereomicroscopy on days 0, 3, 7 and 10 after pellet implantation or alkali injury as well as in flat\u2010mounted corneas via confocal microscopy after the final\n                    <jats:italic>in\u00a0vivo<\/jats:italic>\n                    imaging time point. We observed blood and lymphatic vessel growth in all three models, with the most significant growth occurring from days 0\u20137. Upon\n                    <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>\n                    stimulation, the growth kinetics of blood and lymphatic vessels were similar. Blood vessels exhibited similar growth patterns in\n                    <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>\n                    \u2010 and bFGF\u2010stimulated corneas. Alkali burn injury induced robust angiogenesis and lymphangiogenesis. The intrinsic fluorescence of blood and lymphatic endothelial cells in Prox1\u2013\n                    <jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>\n                    \/Flk1::myr\u2013mCherry mice permitted simultaneous\n                    <jats:italic>in\u00a0vivo<\/jats:italic>\n                    imaging of angiogenesis and lymphangiogenesis. This allowed us to differentiate the processes as well as observe their inter\u2010dependence, and will be valuable in development of therapies targeting angiogenesis and\/or lymphangiogenesis.\n                  <\/jats:p>","DOI":"10.1111\/febs.13234","type":"journal-article","created":{"date-parts":[[2015,2,17]],"date-time":"2015-02-17T08:20:53Z","timestamp":1424161253000},"page":"1458-1467","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Simultaneous\n                    <i>in\u00a0vivo<\/i>\n                    imaging of blood and lymphatic vessel growth in Prox1\u2013\n                    <scp>GFP<\/scp>\n                    \/Flk1::myr\u2013mCherry mice"],"prefix":"10.1111","volume":"282","author":[{"given":"Jimmy","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences Illinois Eye and Ear Infirmary University of Illinois at Chicago IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Dugas\u2010Ford","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences Illinois Eye and Ear Infirmary University of Illinois at Chicago IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences Illinois Eye and Ear Infirmary University of Illinois at Chicago IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patryk","family":"Purta","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences Illinois Eye and Ear Infirmary University of Illinois at Chicago IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyu\u2010Yeon","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences Illinois Eye and Ear Infirmary University of Illinois at Chicago IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young\u2010Kwon","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Surgery Norris Comprehensive Cancer Center Keck School of Medicine University of Southern California Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary E.","family":"Dickinson","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology and Biophysics Baylor College of Medicine Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark I.","family":"Rosenblatt","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences Illinois Eye and Ear Infirmary University of Illinois at Chicago IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Hong","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of 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