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The dramatic amplification of <jats:styled-content style=\"fixed-case\">MITE<\/jats:styled-content> families during evolution is puzzling, because the transposase sources for the vast majority of <jats:styled-content style=\"fixed-case\">MITE<\/jats:styled-content> families are unknown. The yellow fever mosquito genome contains &gt;\u00a0220\u2010Mb <jats:styled-content style=\"fixed-case\">MITE<\/jats:styled-content> sequences; however, transposition activity has not been demonstrated for any of the <jats:styled-content style=\"fixed-case\">MITE<\/jats:styled-content> families. The <jats:italic>Gnome<\/jats:italic> elements are the youngest <jats:styled-content style=\"fixed-case\">MITE<\/jats:styled-content> family in this genome, with at least 116 identical copies. To test whether the putative autonomous element <jats:italic>Ozma<\/jats:italic> is capable of mobilizing <jats:italic>Gnome<\/jats:italic> and its two sibling <jats:styled-content style=\"fixed-case\">MITE<\/jats:styled-content>s, analyses were performed in a yeast transposition assay system. Whereas the wild\u2010type transposase resulted in very low transposition activity, mutations in the region containing a putative nuclear export signal motif resulted in a dramatic (at least 4160\u2010fold) increase in transposition frequency. We have also demonstrated that each residue of the novel <jats:styled-content style=\"fixed-case\">DD<\/jats:styled-content>37E motif is required for the activity of the Ozma transposase. Footprint sequences left at the donor sites suggest that the transposase may cleave between the second and the third nucleotides from the 5' ends of the elements. The excised elements reinsert specifically at dinucleotide \u2018<jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content>\u2019, ~\u00a055% of them in yeast genes. The elements described in this article could potentially be useful as genetic tools for genetic manipulation of mosquitoes.<\/jats:p>","DOI":"10.1111\/febs.13257","type":"journal-article","created":{"date-parts":[[2015,3,5]],"date-time":"2015-03-05T13:34:56Z","timestamp":1425562496000},"page":"1829-1840","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Efficient transposition of the youngest miniature inverted repeat transposable element family of yellow fever mosquito in yeast"],"prefix":"10.1111","volume":"282","author":[{"given":"Isam","family":"Fattash","sequence":"first","affiliation":[{"name":"Department of Biology University of Toronto Mississauga ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia\u2010Ni","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biology University of Toronto Mississauga ON 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