{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T11:45:40Z","timestamp":1791978340342,"version":"4.4.0"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,12,12]],"date-time":"2015-12-12T00:00:00Z","timestamp":1449878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:p>Mice deficient for zinc transporter 7 protein (ZnT7) are mildly zinc deficient with low body weight gain and body fat accumulation. To investigate the underlying mechanism of ZnT7 deficiency in body adiposity, we examined fatty acid composition and insulin sensitivity in visceral (epididymal) and subcutaneous fat pads from <jats:italic>Znt7<\/jats:italic> knockout and control mice. We showed that ZnT7 deficiency had adverse effects on fatty acid metabolism and insulin action in subcutaneous fat but not in epididymal fat in mice, consistent with the ZnT7 protein expression pattern in adipose tissues. Importantly, we found that the expression of ZnT7 protein was induced by lipogenic differentiation and reached a peak when the adipocyte was fully differentiated in mouse 3T3\u2010L1 adipocytes. We demonstrated, using <jats:italic>Znt7<\/jats:italic> knockdown (<jats:italic>Znt7<\/jats:italic><jats:styled-content style=\"fixed-case\">KD<\/jats:styled-content>) 3T3\u2010L1 adipocytes, that reduction in <jats:italic>Znt7<\/jats:italic> expression blunted activations of the signal transduction pathways that regulated both basal and insulin\u2010stimulated glucose uptake in adipocytes, resulting in low glucose uptake and lipid accumulation. The expression of the signaling mediators critical for the initiation of pre\u2010adipocyte differentiation, including Ppar\u03b3 and C\/Ebp\u03b1, appeared not to be affected by <jats:italic>Znt7<\/jats:italic><jats:styled-content style=\"fixed-case\">KD<\/jats:styled-content> in 3T3\u2010L1 adipocytes. These findings strongly suggest a role for ZnT7 in adipocyte lipogenesis.<\/jats:p>","DOI":"10.1111\/febs.13582","type":"journal-article","created":{"date-parts":[[2015,11,2]],"date-time":"2015-11-02T13:51:17Z","timestamp":1446472277000},"page":"378-394","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Zinc transporter 7 deficiency affects lipid synthesis in adipocytes by inhibiting insulin\u2010dependent Akt activation and glucose uptake"],"prefix":"10.1111","volume":"283","author":[{"given":"Surapun","family":"Tepaamorndech","sequence":"first","affiliation":[{"name":"Integrative Genetics and Genomics Graduate Group University of California Davis CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine P.","family":"Kirschke","sequence":"additional","affiliation":[{"name":"Obesity and Metabolism Research Unit USDA\/ARS\/Western Human Nutrition Research Center Davis CA 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