{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T03:40:05Z","timestamp":1785814805415,"version":"3.56.0"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,1,4]],"date-time":"2016-01-04T00:00:00Z","timestamp":1451865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","award":["SR\/SO\/PS\u2010102\/2009"],"award-info":[{"award-number":["SR\/SO\/PS\u2010102\/2009"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:p><jats:italic><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methyltransferase 2<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">DNMT<\/jats:styled-content>2<\/jats:italic>) unlike other members of the cytosine <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methyltransferase gene family has dual substrate specificity and it methylates cytosines in both the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and transfer <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (t<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>). Its role in plants, however, has remained obscure to date. In this study, we demonstrate that <jats:italic><jats:styled-content style=\"fixed-case\">DNMT<\/jats:styled-content>2<\/jats:italic> from <jats:italic>Physcomitrella patens<\/jats:italic> accumulates in a temporal manner under salt and osmotic stress showing maximum accumulation during recovery, i.e. 24 h after plants are transferred to normal growth medium. Therefore, to study its role in stress tolerance, we generated <jats:italic>Pp<jats:styled-content style=\"fixed-case\">DNMT<\/jats:styled-content>2<\/jats:italic> targeted knockout plants (<jats:italic>ppdnmt2<\/jats:italic>ko). Mutant plants show increased sensitivity to salt and osmotic stress and are unable to recover even after 21 days of growth on optimal growth media. <jats:italic>ppdnmt2<\/jats:italic>ko, however, accumulate normal levels of dehydrin\u2010like and small heat shock protein encoding transcripts under stress but show dramatic reduction in levels of t<jats:styled-content style=\"fixed-case\">RNA<jats:sup>A<\/jats:sup><\/jats:styled-content><jats:sup>sp\u2010<\/jats:sup><jats:styled-content style=\"fixed-case\"><jats:sup>GUC<\/jats:sup><\/jats:styled-content>. The levels of t<jats:styled-content style=\"fixed-case\">RNA<jats:sup>A<\/jats:sup><\/jats:styled-content><jats:sup>sp\u2010<\/jats:sup><jats:styled-content style=\"fixed-case\"><jats:sup>GUC<\/jats:sup><\/jats:styled-content>, in contrast, increase ~ 25\u201330\u2010fold in <jats:italic>ppdnmt2<\/jats:italic>ko under non\u2010stress conditions and &gt; 1200\u2010fold in wild\u2010type plants under stress. The role of <jats:italic>Pp<jats:styled-content style=\"fixed-case\">DNMT<\/jats:styled-content>2<\/jats:italic> in modulating biogenesis\/stability of t<jats:styled-content style=\"fixed-case\">RNA<jats:sup>A<\/jats:sup><\/jats:styled-content><jats:sup>sp\u2010<\/jats:sup><jats:styled-content style=\"fixed-case\"><jats:sup>GUC<\/jats:sup><\/jats:styled-content> under salt and osmotic stress is discussed in the light of these observations.<\/jats:p>","DOI":"10.1111\/febs.13611","type":"journal-article","created":{"date-parts":[[2015,12,8]],"date-time":"2015-12-08T17:18:37Z","timestamp":1449595117000},"page":"556-570","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["<i>Physcomitrella patens DNA methyltransferase 2<\/i> is required for recovery from salt and osmotic stress"],"prefix":"10.1111","volume":"283","author":[{"given":"Deepshikha","family":"Arya","sequence":"first","affiliation":[{"name":"University School 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