{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:07:57Z","timestamp":1791407277241,"version":"4.3.3"},"reference-count":57,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,2,26]],"date-time":"2017-02-26T00:00:00Z","timestamp":1488067200000},"content-version":"am","delay-in-days":337,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2016,3,26]],"date-time":"2016-03-26T00:00:00Z","timestamp":1458950400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["AS2614150Q"],"award-info":[{"award-number":["AS2614150Q"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["15K08409"],"award-info":[{"award-number":["15K08409"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000884","name":"Cancer Research Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000884","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:p><jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>28 (disintegrin and metalloproteinase 28), which was originally reported to be lymphocyte\u2010specific, is over\u2010expressed by carcinoma cells and plays a key role in cell proliferation and progression in human lung and breast carcinomas. We studied <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>28 expression in human normal tissues and examined its biological function. By using antibodies specific to <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>28, <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>28 was immunolocalized mainly to epithelial cells in several tissues, including epididymis, bronchus and stomach, whereas lymphocytes in lymph nodes and spleen were negligibly immunostained. <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, immunoblotting and <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> analyses confirmed the expression in these tissues, and low or negligible expression by lymphocytes was found in the lymph node and spleen. C1q was identified as a candidate <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>28\u2010binding protein from a human lung cDNA library by yeast two\u2010hybrid system, and specific binding was demonstrated by binding assays, immunoprecipitation and surface plasmon resonance. C1q treatment of normal bronchial epithelial <jats:styled-content style=\"fixed-case\">BEAS<\/jats:styled-content>\u20102B and <jats:styled-content style=\"fixed-case\">NHBE<\/jats:styled-content> cells, both of which showed low\u2010level expression of <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>28, caused apoptosis through activation of p38 and caspase\u20103, and cell death with autophagy through accumulation of <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>3\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> and autophagosomes, respectively. C1q\u2010induced cell death was attenuated by treatment of the cells with antibodies against the C1q receptor gC1qR\/p33 or cC1qR\/calreticulin. Treatment of C1q with recombinant <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>28 prior to addition to culture media reduced C1q\u2010induced cell death, and knockdown of <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>28 using si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s increased cell death. These data demonstrate that <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>28 is expressed by epithelial cells of several normal organs, and suggest that <jats:styled-content style=\"fixed-case\">ADAM<\/jats:styled-content>28 plays a role in cell survival by suppression of C1q\u2010induced cytotoxicity in bronchial epithelial cells.<\/jats:p>","DOI":"10.1111\/febs.13693","type":"journal-article","created":{"date-parts":[[2016,2,26]],"date-time":"2016-02-26T14:50:19Z","timestamp":1456498219000},"page":"1574-1594","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["<scp>ADAM<\/scp>28 is expressed by epithelial cells in human normal tissues and protects from C1q\u2010induced cell death"],"prefix":"10.1111","volume":"283","author":[{"given":"Yuka","family":"Miyamae","sequence":"first","affiliation":[{"name":"Department of Pathology Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satsuki","family":"Mochizuki","sequence":"additional","affiliation":[{"name":"Department of Pathology Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayuki","family":"Shimoda","sequence":"additional","affiliation":[{"name":"Department of Pathology Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentaro","family":"Ohara","sequence":"additional","affiliation":[{"name":"Department of Pathology Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Abe","sequence":"additional","affiliation":[{"name":"Department of Pathology Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuji","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Pathology Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saiko","family":"Kazuno","sequence":"additional","affiliation":[{"name":"Department of Proteomics and Biomolecular Science Research Support Center Juntendo University Graduate School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Ohtsuka","sequence":"additional","affiliation":[{"name":"Department of Surgery Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Ochiai","sequence":"additional","affiliation":[{"name":"Department of Surgery Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Kitagawa","sequence":"additional","affiliation":[{"name":"Department of Surgery Keio University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasunori","family":"Okada","sequence":"additional","affiliation":[{"name":"Department of Pathology Keio University School of Medicine Tokyo Japan"},{"name":"Department of Pathophysiology for Locomotive and Neoplastic Diseases Juntendo University Graduate School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,3,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2008.08.001"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2008.09.004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1827.2010.02547.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2003.08.001"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1039703"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1548"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2007.00434.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.41.29251"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161209788682424"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21324"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-0377"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djs232"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.10.077"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.07.011"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/5.12.1127"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3480021"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(01)00871-X"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.04.067"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2013.04.014"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00052"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2000.01218.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1078\/0171-2985-00142"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.7.4715"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2260207"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.5.2441"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-06-2218"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M409174200"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.2.667"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.141.9.3177"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.21875"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2009.06494.x"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2010.03.012"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2012.02.001"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.146.10.3356"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.66.3.416"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.161.12.6924"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005755"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M702414200"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.01.022"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1997.4607"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M700158200"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25212"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(90)90014-H"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903215"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.155.5.2614"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2008.150"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2239"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.093575"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.13515"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2004.03.014"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2007.01.005"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00127.2006"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-0023"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1993.tb00372.x"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-0115(00)00095-1"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.5.8140"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2001-0033"}],"container-title":["The FEBS Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ffebs.13693","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ffebs.13693","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.13693","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/febs.13693","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/am-pdf\/10.1111\/febs.13693","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.13693","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T06:25:17Z","timestamp":1694327117000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/febs.13693"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,26]]},"references-count":57,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["10.1111\/febs.13693"],"URL":"https:\/\/doi.org\/10.1111\/febs.13693","archive":["Portico"],"relation":{},"ISSN":["1742-464X","1742-4658"],"issn-type":[{"value":"1742-464X","type":"print"},{"value":"1742-4658","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,26]]},"assertion":[{"value":"2015-10-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-02-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-03-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}