{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:41:38Z","timestamp":1761709298062,"version":"3.37.3"},"reference-count":36,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2016,12,5]],"date-time":"2016-12-05T00:00:00Z","timestamp":1480896000000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31302045"],"award-info":[{"award-number":["31302045"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:p>\u03b2\u2010Lactoglobulin (<jats:styled-content style=\"fixed-case\">BLG<\/jats:styled-content>) is a dominant allergen present in the milk of goats and other ungulates, although it is not found in human breast milk. Thus, the presence of <jats:styled-content style=\"fixed-case\">BLG<\/jats:styled-content> restricts the consumption of goat's milk by humans. In the present study, we examined whether the disruption of the <jats:italic><jats:styled-content style=\"fixed-case\">BLG<\/jats:styled-content><\/jats:italic> gene in goats by homologous recombination (<jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>) reduced <jats:styled-content style=\"fixed-case\">BLG<\/jats:styled-content> content in goat's milk and decreased the allergic response to milk. In one approach, exon 2 of the <jats:italic><jats:styled-content style=\"fixed-case\">BLG<\/jats:styled-content><\/jats:italic> gene was efficiently targeted using <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content> with a <jats:italic><jats:styled-content style=\"fixed-case\">BLG<\/jats:styled-content><\/jats:italic> knockout vector. In a second approach to disrupt <jats:italic><jats:styled-content style=\"fixed-case\">BLG<\/jats:styled-content><\/jats:italic> gene expression and drive exogenous human \u03b1\u2010lactalbumin (<jats:italic><jats:styled-content style=\"fixed-case\">hLA<\/jats:styled-content><\/jats:italic>) gene expression, two <jats:italic><jats:styled-content style=\"fixed-case\">hLA<\/jats:styled-content><\/jats:italic> knock\u2010in constructs were used to target exons 1\u20134 of the <jats:italic><jats:styled-content style=\"fixed-case\">BLG<\/jats:styled-content><\/jats:italic> gene via <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>, and expression of <jats:italic><jats:styled-content style=\"fixed-case\">hLA<\/jats:styled-content><\/jats:italic> was then confirmed in goat mammary epithelial cells <jats:italic>in vitro<\/jats:italic>. The recombinant clones from both approaches were then used for somatic cell nuclear transfer, generating two transgenic goats possessing a <jats:italic><jats:styled-content style=\"fixed-case\">BLG<\/jats:styled-content><\/jats:italic> knockout allele or site\u2010specific <jats:italic><jats:styled-content style=\"fixed-case\">hLA<\/jats:styled-content><\/jats:italic> integration allele. Milk assays demonstrated a reduction in <jats:styled-content style=\"fixed-case\">BLG<\/jats:styled-content> levels in both the <jats:italic><jats:styled-content style=\"fixed-case\">BLG<\/jats:styled-content><\/jats:italic> knockout and <jats:italic><jats:styled-content style=\"fixed-case\">hLA<\/jats:styled-content><\/jats:italic> knock\u2010in goats; furthermore, <jats:styled-content style=\"fixed-case\">hLA<\/jats:styled-content> was present in the <jats:styled-content style=\"fixed-case\">hLA<\/jats:styled-content> knock\u2010in goat's milk. Allergenic analysis in mice indicated that the transgenic goat's milk was less allergenic than wild\u2010type goat's milk. These results support the development of gene\u2010targeted animals as an effective tool for reducing allergic reactions to milk and improving nutrition.<\/jats:p>","DOI":"10.1111\/febs.13950","type":"journal-article","created":{"date-parts":[[2016,12,5]],"date-time":"2016-12-05T05:04:08Z","timestamp":1480914248000},"page":"4600-4613","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Generation of \u03b2\u2010lactoglobulin\u2010modified transgenic goats by homologous recombination"],"prefix":"10.1111","volume":"283","author":[{"given":"Hongmei","family":"Zhu","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Northwest A&amp;F University Yangling Shaanxi China"},{"name":"Key Laboratory of Animal Biotechnology Ministry of Agriculture Northwest A&amp;F University Yangling Shaanxi 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