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PfbA is a conserved surface protein of <jats:italic>S.\u00a0pneumoniae<\/jats:italic> and helps the bacterium to colonize the host by recognizing the extracellular matrix (<jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>) molecule fibronectin, as well as blood proteins like plasminogen and human serum albumin. The crystal structure of <jats:styled-content style=\"fixed-case\">rP<\/jats:styled-content>fbA<jats:sub>150\u2013607<\/jats:sub> revealed it to possess a beta\u2010helical region similar to those of carbohydrate\u2010active enzymes as well as a C\u2010terminal segment that resembles the fibronectin\u2010binding regions of fibronectin\u2010binding proteins. To get more insight into the putative carbohydrate\u2010binding property of PfbA and its binding to various host molecules, we generated three different constructs of PfbA and characterized them by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>, isothermal titration calorimetry and bio\u2010layer interferometry experiments. Importantly, the isothermal titration calorimetry experiments revealed that PfbA binds to different saccharides. Further, <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> and bio\u2010layer interferometry experiments identified that (a) apart from fibronectin and plasminogen, the beta helix of PfbA also binds to other <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content> molecules (b) lysines are not responsible for PfbA's binding to plasminogen, (c) in comparison with native fibrinogen, deglycosylated\u2010fibrinogen exhibits reduced binding affinity towards PfbA implying the importance of sugar molecule\u2010PfbA interaction and (d) the C\u2010terminal region of PfbA binds exclusively to the N\u2010terminal F1 modules of fibronectin. Thus, the results of this study show PfbA to be a versatile multidomain and multiligand\u2010binding protein employing different binding mechanisms. These results could be useful for structure\u2010based designing of inhibitors against PfbA.<\/jats:p>","DOI":"10.1111\/febs.14200","type":"journal-article","created":{"date-parts":[[2017,8,17]],"date-time":"2017-08-17T07:00:24Z","timestamp":1502953224000},"page":"3404-3421","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["<i>Streptococcus\u00a0pneumoniae<\/i> surface protein PfbA is a versatile multidomain and multiligand\u2010binding adhesin employing different binding mechanisms"],"prefix":"10.1111","volume":"284","author":[{"given":"Darwin Selvi Jemima","family":"Beulin","sequence":"first","affiliation":[{"name":"Centre of Advanced Study in Crystallography and Biophysics University of Madras Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepthi","family":"Radhakrishnan","sequence":"additional","affiliation":[{"name":"Centre of Advanced Study in Crystallography and Biophysics University of Madras Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharanya C.","family":"Suresh","sequence":"additional","affiliation":[{"name":"Department of Biotechnology &amp; Microbiology School of Life Sciences Kannur University Palayad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chittalakottu","family":"Sadasivan","sequence":"additional","affiliation":[{"name":"Department of Biotechnology &amp; Microbiology School of Life Sciences Kannur University Palayad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaya","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Oral and Molecular Microbiology Osaka University Graduate School of Dentistry Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigetada","family":"Kawabata","sequence":"additional","affiliation":[{"name":"Department of Oral and Molecular Microbiology Osaka University Graduate School of Dentistry Suita Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karthe","family":"Ponnuraj","sequence":"additional","affiliation":[{"name":"Centre of Advanced Study in Crystallography and Biophysics University of Madras Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a010215"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07190.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2014.06.006"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-007-7125-8"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M807087200"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0067190"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2013.11.035"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.024604-0"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2012.11.030"},{"key":"e_1_2_8_11_1","first-page":"223","volume-title":"ITC\u2010Derived Binding Constants: Using Microgram Quantities of Protein. 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