{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T17:48:57Z","timestamp":1759600137642},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,12,29]],"date-time":"2017-12-29T00:00:00Z","timestamp":1514505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007393","name":"Association Fran\u00e7aise contre les Myopathies","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007393","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:p>Recently, a new population of resident cardiac stem cells (<jats:styled-content style=\"fixed-case\">CSC<\/jats:styled-content>s) positive for the W8B2 marker has been identified. These <jats:styled-content style=\"fixed-case\">CSC<\/jats:styled-content>s are considered to be an ideal cellular source to repair myocardial damage after infarction. However, the electrophysiological profile of these cells has not been characterized yet. We first establish the conditions of isolation and expansion of W8B2<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CSC<\/jats:styled-content>s from human heart biopsies using a magnetic sorting system followed by flow cytometry cell sorting. These cells display a spindle\u2010shaped morphology, are highly proliferative, and possess self\u2010renewal capacity demonstrated by their ability to form colonies. Besides, W8B2<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CSC<\/jats:styled-content>s are positive for mesenchymal markers but negative for hematopoietic and endothelial ones. <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> and immunostaining experiments show that W8B2<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CSC<\/jats:styled-content>s express some early cardiac\u2010specific transcription factors but lack the expression of cardiac\u2010specific structural genes. Using patch clamp in the whole\u2010cell configuration, we show for the first time the electrophysiological signature of <jats:styled-content style=\"fixed-case\">BKC<\/jats:styled-content>a current in these cells. Accordingly, <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and western blotting analysis confirmed the presence of <jats:styled-content style=\"fixed-case\">BKC<\/jats:styled-content>a at both <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and protein levels in W8B2<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CSC<\/jats:styled-content>s. Interestingly, <jats:styled-content style=\"fixed-case\">BKC<\/jats:styled-content>a channel inhibition by paxilline decreased cell proliferation in a concentration\u2010dependent manner and halted cell cycle progression at the G0\/G1 phase. The inhibition of <jats:styled-content style=\"fixed-case\">BKC<\/jats:styled-content>a also decreased the self\u2010renewal capacity but did not affect migration of W8B2<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">CSC<\/jats:styled-content>s. Taken together, our results are consistent with an important role of <jats:styled-content style=\"fixed-case\">BKC<\/jats:styled-content>a channels in cell cycle progression and self\u2010renewal in human cardiac stem cells.<\/jats:p>","DOI":"10.1111\/febs.14352","type":"journal-article","created":{"date-parts":[[2017,12,6]],"date-time":"2017-12-06T16:16:48Z","timestamp":1512577008000},"page":"518-530","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Functional BKCa channel in human resident cardiac stem cells expressing W8B2"],"prefix":"10.1111","volume":"285","author":[{"given":"Oualid","family":"Ayad","sequence":"first","affiliation":[{"name":"Equipe Transferts Ioniques et Rythmicit\u00e9 Cardiaque Laboratoire Signalisation et Transports Ioniques Membranaires CNRS ERL 7368, EA 7349 Universit\u00e9 de Poitiers 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