{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:08:54Z","timestamp":1773659334525,"version":"3.50.1"},"reference-count":55,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,2,14]],"date-time":"2019-02-14T00:00:00Z","timestamp":1550102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:p>Prokaryotic (6\u20104) photolyases branch at the base of the evolution of cryptochromes and photolyases. Prototypical members contain an iron\u2013sulphur cluster which was lost in the evolution of the other groups. In the <jats:italic>Agrobacterium<\/jats:italic> (6\u20104) photolyase PhrB, the repair of <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> lesions containing <jats:styled-content style=\"fixed-case\">UV<\/jats:styled-content>\u2010induced (6\u20104) pyrimidine dimers is stimulated by Mg<jats:sup>2+<\/jats:sup>. We propose that Mg<jats:sup>2+<\/jats:sup> is required for efficient lesion binding and for charge stabilization after electron transfer from the <jats:styled-content style=\"fixed-case\">FADH<\/jats:styled-content><jats:sup>\u2212<\/jats:sup> chromophore to the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> lesion. Furthermore, two highly conserved Asp residues close to the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010binding site are essential for the effect of Mg<jats:sup>2+<\/jats:sup>. Simulations show that two Mg<jats:sup>2+<\/jats:sup> bind to the region around these residues. On the other hand, <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> repair by eukaryotic (6\u20104) photolyases is not increased by Mg<jats:sup>2+<\/jats:sup>. In these photolyases, structurally overlapping regions contain no Asp but positively charged Lys or Arg. During the evolution of photolyases, the role of Mg<jats:sup>2+<\/jats:sup> in charge stabilization and enhancement of DNA binding was therefore taken over by a postiviely charged amino acid. Besides PhrB, another prokaryotic (6\u20104) photolyase from the marine cyanobacterium <jats:italic>Prochlorococcus marinus<\/jats:italic>, Proma<jats:styled-content style=\"fixed-case\">PL<\/jats:styled-content>, which contains no iron\u2013sulphur cluster, was also investigated. This photolyase is stimulated by Mg<jats:sup>2+<\/jats:sup> as well. The evolutionary loss of the iron\u2013sulphur cluster due to limiting iron concentrations can occur in a marine environment as a result of iron deprivation. However, the evolutionary replacement of Mg<jats:sup>2+<\/jats:sup> by a positively charged amino acid is unlikely to occur in a marine environment because the concentration of divalent cations in seawater is always sufficient. We therefore assume that this transition could have occurred in a freshwater environment.<\/jats:p>","DOI":"10.1111\/febs.14770","type":"journal-article","created":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T07:05:07Z","timestamp":1549004707000},"page":"1765-1779","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Two aspartate residues close to the lesion binding site of <i>Agrobacterium<\/i> (6\u20104) photolyase are required for Mg<sup>2+<\/sup> stimulation of <scp>DNA<\/scp> repair"],"prefix":"10.1111","volume":"286","author":[{"given":"Hongju","family":"Ma","sequence":"first","affiliation":[{"name":"Botanical Institute Karlsruhe Institute of Technology Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Holub","sequence":"additional","affiliation":[{"name":"Department for Theoretical Chemical Biology Institute for Physical Chemistry Karlsruhe Institute for Technology 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