{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T18:29:28Z","timestamp":1782930568868,"version":"3.54.5"},"reference-count":56,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,2,18]],"date-time":"2019-02-18T00:00:00Z","timestamp":1550448000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["IFCT2015"],"award-info":[{"award-number":["IFCT2015"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PEst\u2010OE\/SAU\/UI0215\/2014"],"award-info":[{"award-number":["PEst\u2010OE\/SAU\/UI0215\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["POCI\/COMPETE2020"],"award-info":[{"award-number":["POCI\/COMPETE2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BBB\u2010BQB\/1368\/2014"],"award-info":[{"award-number":["PTDC\/BBB\u2010BQB\/1368\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BIM\u2010MET\/4712\/2014"],"award-info":[{"award-number":["PTDC\/BIM\u2010MET\/4712\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/103105\/2014"],"award-info":[{"award-number":["SFRH\/BD\/103105\/2014"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/109284\/2015"],"award-info":[{"award-number":["SFRH\/BD\/109284\/2015"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2019,4]]},"abstract":"The process that allows cells to control their pH<\/jats:styled-content> and bicarbonate levels is essential for ionic and metabolic equilibrium. Carbonic anhydrases (CA<\/jats:styled-content>s) catalyse the conversion of CO<\/jats:styled-content>2<\/jats:sub> to and H+<\/jats:sup> and are thus essential for this process. Herein, we inhibited CA<\/jats:styled-content>s with acetazolamide \u2013 ACT<\/jats:styled-content> and SLC<\/jats:styled-content>\u20100111 \u2013 to study their involvement in the metabolism, mitochondrial potential, mitochondrial biogenesis and lipid metabolism of human Sertoli cells (hSC<\/jats:styled-content>s), obtained from biopsies from men with conserved spermatogenesis. We were able to identify three isoforms of CA<\/jats:styled-content>s, one mitochondrial isoform (CA VB<\/jats:styled-content>) and two cell membrane\u2010bound isoforms (CA IX<\/jats:styled-content> and CA XII<\/jats:styled-content>) in hSC<\/jats:styled-content>s. When assessing the expression of markers for mitochondrial biogenesis, we observed a decrease in HIF<\/jats:styled-content>\u20101\u03b1, SIRT<\/jats:styled-content>1, PGC<\/jats:styled-content>1\u03b1 and NRF<\/jats:styled-content>\u20101 mRNA<\/jats:styled-content>s after all CA<\/jats:styled-content>s were inhibited, resulting in decreased mitochondrial DNA<\/jats:styled-content> copy numbers. This was followed by an increased production of lactate and alanine in the same conditions. In addition, consumption of glucose was maintained after inhibition of all CA<\/jats:styled-content>s in hSC<\/jats:styled-content>s. These results indicate a reduced conversion of pyruvate to acetyl\u2010coA, possibly due to decreased mitochondrial function, caused by CA<\/jats:styled-content> inhibition in hSC<\/jats:styled-content>s. Inhibition of CA<\/jats:styled-content>s also caused alterations in lipid metabolism, since we detected an increased expression of hormone\u2010sensitive lipase (HSL<\/jats:styled-content>) in hSC<\/jats:styled-content>s. Our results suggest that CA<\/jats:styled-content>s are essential for mitochondrial biogenesis, glucose and lipid metabolism in hSC<\/jats:styled-content>s. This is the first report showing that CA<\/jats:styled-content>s play an essential role in hSC<\/jats:styled-content> metabolic dynamics, being involved in mitochondrial biogenesis and controlling lactate production.<\/jats:p>","DOI":"10.1111\/febs.14779","type":"journal-article","created":{"date-parts":[[2019,2,6]],"date-time":"2019-02-06T13:22:46Z","timestamp":1549459366000},"page":"1393-1406","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Carbonic anhydrases are involved in mitochondrial biogenesis and control the production of lactate by human Sertoli cells"],"prefix":"10.1111","volume":"286","author":[{"given":"Raquel L.","family":"Bernardino","sequence":"first","affiliation":[{"name":"Department of Microscopy Laboratory of Cell Biology Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB) University of Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u00e2nia R.","family":"Dias","sequence":"additional","affiliation":[{"name":"Department of Microscopy Laboratory of Cell Biology Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB) University of Porto Portugal"},{"name":"University of Beira Interior Covilh\u00e3 Portugal"},{"name":"LAQV\/REQUINTE \u2013 Laboratory of Bromatology and Hydrology Faculty of Pharmacy University of Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno P.","family":"Moreira","sequence":"additional","affiliation":[{"name":"Department of Microscopy Laboratory of Cell Biology Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB) University of Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Cunha","sequence":"additional","affiliation":[{"name":"Centre for Reproductive Genetics Prof. Alberto Barros Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alberto","family":"Barros","sequence":"additional","affiliation":[{"name":"Centre for Reproductive Genetics Prof. Alberto Barros Porto Portugal"},{"name":"Department of Genetics Faculty of Medicine University of Porto Portugal"},{"name":"i3S\u2010 Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Universidade do Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsa","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Microscopy Laboratory of Cell Biology Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB) University of Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rio","family":"Sousa","sequence":"additional","affiliation":[{"name":"Department of Microscopy Laboratory of Cell Biology Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB) University of Porto Portugal"},{"name":"Centre for Reproductive Genetics Prof. Alberto Barros Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco G.","family":"Alves","sequence":"additional","affiliation":[{"name":"Department of Microscopy Laboratory of Cell Biology Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB) University of Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4989-5699","authenticated-orcid":false,"given":"Pedro F.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Microscopy Laboratory of Cell Biology Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB) University of Porto Portugal"},{"name":"Department of Genetics Faculty of Medicine University of Porto Portugal"},{"name":"i3S\u2010 Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade Universidade do Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,2,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/advan.00045.2004"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/138161208783877884"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.455"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543776.10.5.575"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm0612057"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2467"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.10025"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/scdb.1998.0203"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.018648"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/taap.1994.1210"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1971.sp009400"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.24163"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.13.7608"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.4.1677"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00232-010-9294-x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod49.6.1215"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1250769"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00036.2005"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.2174\/15672050113109990200"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/boc.201500094"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00232-013-9601-4"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-015-2481-4"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0496-470"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64038-2"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm050483n"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.64.070195.002111"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543776.2013.790957"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64048-5"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1981.61.2.296"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00232-008-9139-z"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2000.80.2.681"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21325"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.2174\/2212796807999131128125510"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80611-X"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501485200"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-2760(01)00457-X"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/444151a"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.070169"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2006.01.012"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42809-2"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0860011"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(83)90231-X"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1982.tb06675.x"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)01185-0"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.2.787"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)85601-6"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.52.5.1255"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1999.276.2.R559"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1080\/07357900601062321"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7698-0_1"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.3109\/14756366.2013.773994"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm101541x"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2012.06.002"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-08-0363"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1640"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"}],"container-title":["The FEBS Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.14779","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/febs.14779","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.14779","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T19:20:25Z","timestamp":1694373625000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/febs.14779"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,18]]},"references-count":56,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1111\/febs.14779"],"URL":"https:\/\/doi.org\/10.1111\/febs.14779","archive":["Portico"],"relation":{},"ISSN":["1742-464X","1742-4658"],"issn-type":[{"value":"1742-464X","type":"print"},{"value":"1742-4658","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,18]]},"assertion":[{"value":"2018-07-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}