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The eccentric force\u00a0:\u00a0isometric force (ECC<\/jats:styled-content>\u00a0:\u00a0ISO<\/jats:styled-content>), expression level of utrophin, fiber type distribution, and sarcoendoplasmic reticulum calcium ATPase expression are factors that differ between muscles and may contribute to the sensitivity of mdx<\/jats:italic> skeletal muscle to ECC<\/jats:styled-content>. Here, we confirm that the Sol of mdx<\/jats:italic> mice loses only 13% force compared to 87% in the extensor digitorum longus (EDL<\/jats:styled-content>) following 10 ECC<\/jats:styled-content> of isolated muscles. The Sol has a greater proportion of fibers expressing Type I myosin heavy chain (MHC) and expresses 2.3\u2010fold more utrophin compared to the EDL<\/jats:styled-content>. To examine the effect of ECC<\/jats:styled-content> : ISO<\/jats:styled-content>, we show that the mdx<\/jats:italic> Sol is insensitive to ECC<\/jats:styled-content> at ECC<\/jats:styled-content> : ISO<\/jats:styled-content> up to 230\u00a0\u00b1\u00a015%. We show that the peroneus longus (PL) muscle presents with similar ECC<\/jats:styled-content> : ISO<\/jats:styled-content> compared to the EDL<\/jats:styled-content>, intermediate force loss (68%) following 10 ECC<\/jats:styled-content>, and intermediate fiber type distribution and utrophin expression relative to EDL<\/jats:styled-content> and Sol. The combined absence of utrophin and dystrophin in mdx<\/jats:italic>\/utrophin\u2212\/\u2212<\/jats:sup> mice rendered the Sol only partially susceptible to ECC<\/jats:styled-content> and exacerbated force loss in the EDL<\/jats:styled-content> and PL<\/jats:styled-content>. Most interestingly, the expression levels of cytoplasmic \u03b2\u2010 and \u03b3\u2010actins correlate inversely with a given muscle's sensitivity to ECC<\/jats:styled-content>; EDL<\/jats:styled-content>\u00a0<\u00a0PL<\/jats:styled-content>\u00a0<\u00a0Sol. Our data indicate that fiber type, utrophin, and cytoplasmic actin expression all contribute to the differential sensitivities of mdx<\/jats:italic>EDL<\/jats:styled-content>, PL<\/jats:styled-content>, and Sol muscles to ECC<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/febs.14831","type":"journal-article","created":{"date-parts":[[2019,4,3]],"date-time":"2019-04-03T15:18:14Z","timestamp":1554304694000},"page":"2562-2576","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Variable cytoplasmic actin expression impacts the sensitivity of different dystrophin\u2010deficient mdx<\/i> skeletal muscles to eccentric contraction"],"prefix":"10.1111","volume":"286","author":[{"given":"Angus","family":"Lindsay","sequence":"first","affiliation":[{"name":"Division of Rehabilitation Science and Division of Physical Therapy Department of Rehabilitation Medicine University of Minnesota Minneapolis MN USA"},{"name":"Department 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