{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T06:39:46Z","timestamp":1778654386871,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2019,6,28]],"date-time":"2019-06-28T00:00:00Z","timestamp":1561680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31671220"],"award-info":[{"award-number":["31671220"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31871178"],"award-info":[{"award-number":["31871178"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:p>The formation and hydrolysis of hepatic retinyl esters (RE) is a key process in\u00a0maintaining serum retinol homeostasis. During vitamin A deficiency, the activity of RE hydrolases (REH) in liver increases to cope with the reduced dietary vitamin A intake. However, it remains unclear which REH is the main enzyme responsible for RE hydrolysis in the liver under physiological conditions. Our previous studies have shown that pancreatic lipase\u2010related protein 2 (PLRP2) is conditionally expressed in the liver and may be involved in the hydrolysis of hepatic RE. In the current study, we generated <jats:italic>Plrp2<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice using transcription activator\u2010like effector nuclease technology to investigate the role of PLRP2 in the metabolism of hepatic RE. Compared with the mice fed normal diet, the hepatic REH activity of wild\u2010type (WT) mice fed vitamin A\u2010deficient diet (VAD) increased significantly, while this activity did not increase in <jats:italic>Plrp2<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice fed VAD. <jats:italic>Plrp2<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice showed higher residual RE content in liver and lower serum retinol level, compared with WT mice fed VAD. Hepatic metabolic profiling from <jats:sup>1<\/jats:sup>H NMR\u2010based metabolomics suggested that <jats:italic>Plrp2<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice were more sensitive to VAD. Docking analysis and enzyme activity assay revealed that retinyl palmitate was the substrate with higher affinity for PLRP2. Our results indicate that <jats:italic>Plrp2<\/jats:italic> can be activated in the liver and is responsible for the increased REH activity in the liver of mice fed VAD.<\/jats:p>","DOI":"10.1111\/febs.14958","type":"journal-article","created":{"date-parts":[[2019,6,14]],"date-time":"2019-06-14T13:23:59Z","timestamp":1560518639000},"page":"4232-4244","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Pancreatic lipase\u2010related protein 2 is responsible for the increased hepatic retinyl ester hydrolase activity in vitamin A\u2010deficient mice"],"prefix":"10.1111","volume":"286","author":[{"given":"Yan","family":"Gao","sequence":"first","affiliation":[{"name":"Center for Molecular Metabolism Nanjing University of Science &amp; Technology China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiling","family":"Lu","sequence":"additional","affiliation":[{"name":"Center for Molecular Metabolism Nanjing University of Science &amp; Technology China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Sun","sequence":"additional","affiliation":[{"name":"Center for Molecular Metabolism Nanjing University of Science &amp; Technology China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Yang","sequence":"additional","affiliation":[{"name":"Center for Molecular Metabolism Nanjing University of Science &amp; Technology China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junhao","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Molecular Metabolism Nanjing University of Science &amp; Technology China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenhao","family":"Ge","sequence":"additional","affiliation":[{"name":"Center for Molecular Metabolism Nanjing University of Science &amp; Technology China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunxia","family":"Yang","sequence":"additional","affiliation":[{"name":"Center for Molecular Metabolism Nanjing University of Science &amp; Technology China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Center for Molecular Metabolism Nanjing University of Science &amp; Technology China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xi","family":"Xu","sequence":"additional","affiliation":[{"name":"Center for Molecular Metabolism Nanjing University of Science &amp; Technology China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianfa","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Molecular Metabolism Nanjing University of Science &amp; Technology China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,6,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198404193101605"},{"key":"e_1_2_9_3_1","first-page":"71","article-title":"Vitamin A \u2010 functions, dietary requirements and safety in humans","volume":"67","author":"Gerster H","year":"1997","journal-title":"Int J Vitam Nutr Res"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2016.1160362"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2003.10.002"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/neu.20242"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.5858\/2007-131-1728-HSCALF"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1042\/CBI20100321"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN2002753"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2002.03121.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi981679a"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi981680"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)89425-5"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/130.2.340S"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2005.12.013"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(00)01184-6"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04368"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M010082"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.47.31215"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/117.12.2066"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.16.5878"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M014290"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu9010013"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.110.005777"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/134.1.132"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/1028415X.2000.11747314"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.06-7964com"},{"key":"e_1_2_9_28_1","first-page":"3","article-title":"Effect of undernutrition and vitamin A deficiency on the phospholipid composition of rat tissues at 21 days of age. \u2013 I. 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