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These sensors activate a number of pathways to reduce the stress and facilitate cell survival. While much is known about the mechanisms involved that modulate apoptosis during chronic stress, less is known about the transition between the prosurvival and proapoptotic factors that determine cell fate. Here, we employed a genetic screen that utilized three different pharmacological stressors to induce ER stress in a human\u2010immortalized airway epithelial cell line, immortalized human bronchial epithelial cells. We followed the stress responses over an 18\u2010h time course and utilized real\u2010time monitoring of cell survival, next\u2010generation sequencing, and quantitative real\u2010time PCR to identify and validate genes that were upregulated with all three commonly employed ER stressors, inhibitor of calpain 1, tunicamycin, and thapsigargin. growth arrest and DNA damage\u2010inducible alpha (<jats:italic>GADD45A<\/jats:italic>), a proapoptotic factor, and regulator of calcineurin 1 (<jats:italic>RCAN1<\/jats:italic>) mRNAs were identified and verified by showing that small interfering RNA (siRNA) knockdown of <jats:italic>GADD45A<\/jats:italic> decreased CCAAT\u2010enhancer\u2010binding protein homologous protein (a.k.a <jats:italic>DDIT3<\/jats:italic>), BCL2\u2010binding component 3 (a.k.a. BBC3), and phorbol\u201012\u2010myristate\u201013\u2010acetate\u2010induced protein 1 expression, 3 proapoptotic factors, and increased cell viability during ER stress conditions, whereas siRNA knockdown of <jats:italic>RCAN<\/jats:italic>1 dramatically decreased cell viability. These results suggest that the relative levels of these two genes regulate cell fate decisions during ER stress independent of the type of ER stressor.<\/jats:p>","DOI":"10.1111\/febs.15195","type":"journal-article","created":{"date-parts":[[2019,12,27]],"date-time":"2019-12-27T13:35:28Z","timestamp":1577453728000},"page":"2923-2947","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Genome\u2010wide mRNA profiling identifies <i>RCAN1<\/i> and <i>GADD45A<\/i> as regulators of the transitional switch from survival to apoptosis during ER stress"],"prefix":"10.1111","volume":"287","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2864-6757","authenticated-orcid":false,"given":"Rafal","family":"Bartoszewski","sequence":"first","affiliation":[{"name":"Department of Biology and Pharmaceutical Botany Medical University of Gdansk 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