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Deletion of <jats:italic>MSMEG_1285<\/jats:italic> enhances <jats:italic>Mycobacterium\u00a0smegmatis<\/jats:italic> resistance to penicillin antibiotics, a phenotype that is exacerbated by the additional deletion of <jats:italic>hbhA<\/jats:italic>. This also led to a substantial decrease in the amounts of porins in the cell wall, which are necessary for the import of small and hydrophilic \u03b2\u2010lactams. Deletion of both <jats:italic>MSMEG_1285<\/jats:italic> and <jats:italic>hbhA<\/jats:italic> provoked an over\u2010representation of several enzymes involved in peptidoglycan degradation. Thus, we propose that MSMEG_1285 is part of a protein scaffold, which also involves PonA1 and HbhA, and that it is responsible for the tight regulation of peptidoglycan hydrolysis. This study provides a better understanding of the mycobacterial physiology, which is an essential step for strengthening the action of drugs that specifically target peptidoglycan biosynthesis.<\/jats:p>","DOI":"10.1111\/febs.15232","type":"journal-article","created":{"date-parts":[[2020,1,29]],"date-time":"2020-01-29T11:46:31Z","timestamp":1580298391000},"page":"4415-4426","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Protein scaffold involving MSMEG_1285 maintains cell wall organization and mediates penicillin sensitivity in mycobacteria"],"prefix":"10.1111","volume":"287","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6902-5917","authenticated-orcid":false,"given":"Romain","family":"Veyron\u2010Churlet","sequence":"first","affiliation":[{"name":"U1019 \u2013 UMR9017 \u2013 CIIL \u2013 Center for Infection and Immunity of Lille CNRS Inserm CHU Lille Institut Pasteur de Lille Universit\u00e9 de Lille 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