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Transplanted rodent gastrulating embryos\u2010proper (primitive streak and three germ layers) are the source of teratoma\/teratocarcinoma tumors. Human primitive\u2010streak remnants develop sacrococcygeal teratomas that may recur even when benign (well differentiated). To screen for unknown <jats:styled-content style=\"fixed-case\">VPA<\/jats:styled-content> impact on teratoma\u2010type tumors, we used original 2\u2010week embryo\u2010derived teratoma <jats:italic>in\u00a0vitro<\/jats:italic> biological system completed by a spent media metabolome analysis. Gastrulating 9.5\u2010day\u2010old rat embryos\u2010proper were cultivated in Eagle's minimal essential medium (<jats:styled-content style=\"fixed-case\">MEM<\/jats:styled-content>) with 50% rat serum (controls) or with the addition of 2\u00a0m<jats:sc>m<\/jats:sc><jats:styled-content style=\"fixed-case\">VPA<\/jats:styled-content>. Spent media metabolomes were analyzed by <jats:styled-content style=\"fixed-case\">FTIR<\/jats:styled-content>. Compared to controls, <jats:styled-content style=\"fixed-case\">VPA<\/jats:styled-content> acetylated histones; significantly diminished overall teratoma growth, impaired survival, increased the apoptotic index, and decreased proliferation index and incidence of differentiated tissues (e.g., neural tissue). Control teratomas continued to grow and differentiate for 14\u00a0days in isotransplants <jats:italic>in\u00a0vivo<\/jats:italic>, but <jats:italic>in\u00a0vitro\u00a0<\/jats:italic><jats:styled-content style=\"fixed-case\">VPA<\/jats:styled-content>\u2010treated teratomas resorbed. Principal component analysis of <jats:styled-content style=\"fixed-case\">FTIR<\/jats:styled-content> results showed that spent media metabolomes formed well\u2010separated clusters reflecting the treatment and day of cultivation. In metabolomes of <jats:styled-content style=\"fixed-case\">VPA<\/jats:styled-content>\u2010treated teratomas, we found elevation of previously described histone acetylation biomarkers [amide I \u03b1\u2010helix and A(CH<jats:sub>3<\/jats:sub>)\/A(CH<jats:sub>2<\/jats:sub>)]) with apoptotic biomarkers within the amide I region for \u03b2\u2010sheets, and unordered and <jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>2<\/jats:sub> vibrations of lipids. <jats:styled-content style=\"fixed-case\">VPA<\/jats:styled-content> may be proposed for therapy of the undifferentiated component of teratoma tumors and this biological system completed by metabolome analysis, for a faster dual screening of antitumor\/embryotoxic 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