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We have previously shown that hypoxia\u2010inducible factor\u20101\u03b1 (Hif\u20101\u03b1) stabilisation helps the host to clear mycobacterial infection via neutrophil activation. However, Hif\u20101\u03b1 stabilisation has also been implicated in chronic inflammatory diseases caused by prolonged neutrophilic inflammation. Comorbid infection and inflammation can be found together in disease settings, and it remains unclear whether Hif\u20101\u03b1 stabilisation would be beneficial in a holistic disease setting. Here, we set out to understand the effects of Hif\u20101\u03b1 on neutrophil behaviour in a comorbid setting by combining two well\u2010characterised<jats:italic>in\u00a0vivo<\/jats:italic>zebrafish models \u2013 TB infection (<jats:italic>Mycobacterium\u00a0marinum<\/jats:italic>infection) and sterile injury (tailfin transection). Using a local Mm infection near to the tailfin wound site caused neutrophil migration between the two sites that was reduced during Hif\u20101\u03b1 stabilisation. During systemic Mm infection, wounding leads to increased infection burden, but the protective effect of Hif\u20101\u03b1 stabilisation remains. Our data indicate that Hif\u20101\u03b1 stabilisation alters neutrophil migration dynamics between comorbid sites and that the protective effect of Hif\u20101\u03b1 against Mm is maintained in the presence of inflammation, highlighting its potential as a host\u2010derived target against TB infection.<\/jats:p>","DOI":"10.1111\/febs.15433","type":"journal-article","created":{"date-parts":[[2020,6,2]],"date-time":"2020-06-02T20:36:24Z","timestamp":1591130184000},"page":"3925-3943","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Hif\u20101alpha stabilisation is protective against infection in zebrafish comorbid models"],"prefix":"10.1111","volume":"287","author":[{"given":"Yves","family":"Schild","sequence":"first","affiliation":[{"name":"The Bateson Centre University of Sheffield Sheffield UK"},{"name":"Department of Infection, Immunity and Cardiovascular Disease University of Sheffield Sheffield 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