{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:16:03Z","timestamp":1791317763106,"version":"4.3.1"},"reference-count":51,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000361","name":"Diabetes UK","doi-asserted-by":"publisher","award":["17\/0005678"],"award-info":[{"award-number":["17\/0005678"]}],"id":[{"id":"10.13039\/501100000361","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:p>\n                    Several studies have shown a clear association between periodontal disease and increased risk of cardiovascular disease.\n                    <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    (\n                    <jats:italic>Pg<\/jats:italic>\n                    ), a key oral pathogen, and its cell surface\u2010expressed gingipains, induce oedema in a zebrafish larvae infection model although the mechanism of these vascular effects is unknown. Here, we aimed to determine whether\n                    <jats:italic>Pg<\/jats:italic>\n                    \u2010induced vascular damage is mediated by gingipains.\n                    <jats:italic>In\u00a0vitro<\/jats:italic>\n                    , human endothelial cells from different vascular beds were invaded by wild\u2010type (W83) but not gingipain\u2010deficient (\u0394K\/R\u2010ab)\n                    <jats:italic>Pg<\/jats:italic>\n                    . W83 infection resulted in increased endothelial permeability as well as decreased cell surface abundance of endothelial adhesion molecules PECAM\u20101 and VE\u2010cadherin compared to infection with \u0394K\/R\u2010ab. In agreement, when transgenic zebrafish larvae expressing fluorescently labelled PECAM\u20101 or VE\u2010cadherin were systemically infected with W83 or \u0394K\/R\u2010ab, a significant reduction in adhesion molecule fluorescence was observed specifically in endothelium proximal to W83 bacteria through a gingipain\u2010dependent mechanism. Furthermore, this was associated with increased vascular permeability\n                    <jats:italic>in\u00a0vivo<\/jats:italic>\n                    when assessed by dextran leakage microangiography. These data are the first to show that\n                    <jats:italic>Pg<\/jats:italic>\n                    directly mediates vascular damage\n                    <jats:italic>in\u00a0vivo<\/jats:italic>\n                    by degrading PECAM\u20101 and VE\u2010cadherin. Our data provide a molecular mechanism by which\n                    <jats:italic>Pg<\/jats:italic>\n                    might contribute to cardiovascular disease.\n                  <\/jats:p>","DOI":"10.1111\/febs.15486","type":"journal-article","created":{"date-parts":[[2020,7,18]],"date-time":"2020-07-18T07:50:13Z","timestamp":1595058613000},"page":"1479-1495","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":76,"title":["Mechanisms of vascular damage by systemic dissemination of the oral pathogen\n                    <i>Porphyromonas gingivalis<\/i>"],"prefix":"10.1111","volume":"288","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1318-8885","authenticated-orcid":false,"given":"Cher","family":"Farrugia","sequence":"first","affiliation":[{"name":"School of Clinical Dentistry University of Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham P.","family":"Stafford","sequence":"additional","affiliation":[{"name":"School of Clinical Dentistry University of Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Potempa","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Biochemistry, Biophysics and Biotechnology Jagiellonian University Krak\u00f3w Poland"},{"name":"Department of Oral Immunity and Infectious Diseases University of Louisville School of Dentistry KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert N.","family":"Wilkinson","sequence":"additional","affiliation":[{"name":"School of Life Sciences Medical School University of Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Infection, Immunity and Cardiovascular Disease Medical School University of Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9724-122X","authenticated-orcid":false,"given":"Craig","family":"Murdoch","sequence":"additional","affiliation":[{"name":"School of Clinical Dentistry University of Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Widziolek","sequence":"additional","affiliation":[{"name":"School of Clinical Dentistry University of Sheffield UK"},{"name":"Department of Microbiology Faculty of Biochemistry, Biophysics and Biotechnology Jagiellonian University Krak\u00f3w Poland"},{"name":"Department of Evolutionary Immunology Institute of Zoology and Biomedical Research Faculty of Biology Jagiellonian University Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2013.1340019"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.5.731"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.38348"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.28.1.27"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3402\/jom.v8.33029"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2009.05.002"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2337"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0089-x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2873"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00053"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389203033487036"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02004-07"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12060"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.11-S.2106"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2011.01717.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/20002297.2017.1281562"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000002067"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.11.5792-5798.1999"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0300-9475.2004.01511.x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.1.268-276.2002"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00661.x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.10.5910-5918.2004"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.3.1386-1398.2005"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.3.1543-1552.2005"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.017897"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2008.03087.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep36023"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.104.004366"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-013-1779-3"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.3.1661-1672.2006"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000155018.67835.1a"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.013383-0"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/825397"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.09.18"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052606"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3402\/jom.v7.28788"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.10.3878-3885.1995"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2014.01.004"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01013-06"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1159\/000024499"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.06098-11"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsim.2004.12.003"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-003-4071-z"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-018-1156-1"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00038.2016"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2015.00365"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01572-07"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0097811"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/ftx003"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08590-7"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.401"}],"container-title":["The FEBS Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ffebs.15486","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.15486","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/febs.15486","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.15486","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T15:50:30Z","timestamp":1693669830000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/febs.15486"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8]]},"references-count":51,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,3]]}},"alternative-id":["10.1111\/febs.15486"],"URL":"https:\/\/doi.org\/10.1111\/febs.15486","archive":["Portico"],"relation":{},"ISSN":["1742-464X","1742-4658"],"issn-type":[{"value":"1742-464X","type":"print"},{"value":"1742-4658","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8]]},"assertion":[{"value":"2020-04-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}