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Recent work shows that subtle temperature changes in the physiologically relevant temperature range can dramatically alter kinase activity and specificity. We argue that temperature is an essential factor that should be considered in inhibitor screening campaigns. In many cases, high\u2010throughput screening is performed at room temperature or 30\u00a0\u00b0C, which may lead to many false positives and false negatives when evaluating potential inhibitors in the physiological temperature range. As one example, we discuss a new antimalaria compound that inhibits the highly temperature\u2010sensitive kinase CLK3 (CDC2\u2010like kinase 3) from <jats:italic>Plasmodium\u00a0falciparum<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/febs.15564","type":"journal-article","created":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T20:41:37Z","timestamp":1600461697000},"page":"3148-3153","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Temperature does matter\u2014an additional dimension in kinase inhibitor development"],"prefix":"10.1111","volume":"288","author":[{"given":"Miriam","family":"Strauch","sequence":"first","affiliation":[{"name":"Laboratory of RNA Biochemistry Institute of Chemistry and Biochemistry Freie Universitaet Berlin 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