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Using a high\u2010throughput approach of affinity\u2010profiling against the full human PDZome, we identified sixteen human PDZ binders of SARS\u2010CoV\u20102 proteins E, 3A, and N showing significant interactions with dissociation constants values ranging from 3 to 82\u00a0\u03bc\n                    <jats:sc>m<\/jats:sc>\n                    . Six of them (TJP1, PTPN13, HTRA1, PARD3, MLLT4, LNX2) are also recognized by SARS\u2010CoV while three (NHERF1, MAST2, RADIL) are specific to SARS\u2010CoV\u20102 E protein. Most of these SARS\u2010CoV\u20102 protein partners are involved in cellular junctions\/polarity and could be also linked to evasion mechanisms of the immune responses during viral infection. Among the binders of the SARS\u2010CoV\u20102 proteins E, 3a, or N, seven significantly affect viral replication under knock down gene expression in infected cells. This PDZ profiling identifying human proteins potentially targeted by SARS\u2010CoV\u20102 can help to understand the multifactorial severity of COVID19 and to conceive effective anti\u2010coronaviral agents for therapeutic purposes.\n                  <\/jats:p>","DOI":"10.1111\/febs.15881","type":"journal-article","created":{"date-parts":[[2021,4,17]],"date-time":"2021-04-17T13:11:43Z","timestamp":1618665103000},"page":"5148-5162","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":73,"title":["Host PDZ\u2010containing proteins targeted by SARS\u2010CoV\u20102"],"prefix":"10.1111","volume":"288","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5066-0435","authenticated-orcid":false,"given":"C\u00e9lia","family":"Caillet\u2010Saguy","sequence":"first","affiliation":[{"name":"Institut Pasteur Unit\u00e9 R\u00e9cepteurs\u2010Canaux UMR CNRS 3571 Paris 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