{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T00:22:03Z","timestamp":1776039723340,"version":"3.50.1"},"reference-count":110,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2022,3,11]],"date-time":"2022-03-11T00:00:00Z","timestamp":1646956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100017220","name":"Regional Centre for Biotechnology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017220","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:p>\n                    Apicomplexans such as the malaria parasite\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    possess a unique organelle known as the apicoplast that has its own circular genome. The apicoplast genome is AT rich and is subjected to oxidative stress from the byproducts of the normal biochemical pathways that operate in the apicoplast. It is expected that oxidative stress will lead to the appearance of DNA lesions such as 2\u2010hydroxydeoxyadenine, thymine glycol, and 8\u2010oxodeoxyguanine in the apicoplast genome. The apicoplast genome is replicated by the DNA polymerase activity present in the Pfprex enzyme. We have named the polymerase module of Pfprex as PfpPol and the enzyme belongs to the A family of DNA polymerases. Similar to other members of this family, PfpPol also exhibits high fidelity of DNA synthesis. We show that this enzyme is also capable of carrying out translesion DNA synthesis past common DNA lesions that arise due to oxidative stress. The residues N505 and Y509 from the fingers sub\u2010domain, which are unique to PfpPol, play an important role in the ability of PfpPol to bypass the three lesions. The observed lesion\u2010bypass ability of the Pfprex enzyme will minimize the adverse effects of oxidative stress on the apicoplast genome of the malaria parasite. These findings also have implications regarding the evolution of the machinery responsible for replication of organellar genomes.\n                  <\/jats:p>","DOI":"10.1111\/febs.16414","type":"journal-article","created":{"date-parts":[[2022,2,27]],"date-time":"2022-02-27T00:58:11Z","timestamp":1645923491000},"page":"5218-5240","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Pfprex from\n                    <i>Plasmodium falciparum<\/i>\n                    can bypass oxidative stress\u2010induced DNA lesions"],"prefix":"10.1111","volume":"289","author":[{"given":"Minakshi","family":"Sharma","sequence":"first","affiliation":[{"name":"Regional Centre for Biotechnology  Faridabad India"},{"name":"Kalinga Institute of Industrial Technology  Bhubaneshwar India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0677-9444","authenticated-orcid":false,"given":"Deepak 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