{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T02:53:43Z","timestamp":1767840823415,"version":"3.49.0"},"reference-count":39,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2022,6,13]],"date-time":"2022-06-13T00:00:00Z","timestamp":1655078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010226","name":"Department of Education of Guangdong Province","doi-asserted-by":"publisher","award":["2017KQNCX070"],"award-info":[{"award-number":["2017KQNCX070"]}],"id":[{"id":"10.13039\/501100010226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2020A1515011532"],"award-info":[{"award-number":["2020A1515011532"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81602630"],"award-info":[{"award-number":["81602630"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:p>Cancer cells are characterized by altered energetic metabolism with increasing glucose uptake. F806, a 16\u2010membered macrodiolide analogue, has anti\u2010tumour effects on oesophageal squamous cell carcinoma (ESCC) cells. However, its precise anti\u2010tumour mechanism remains unclear. Here, metascape analysis of our previous quantitative proteomics data showed that F806 induced glucose starvation response and inhibited energy production in ESCC cells. The reduced glucose uptake and ATP production were further validated by the fluorescent methods, using glucose\u2010conjugated bioprobe Glu\u20101\u2010O\u2010DCSN, and the bioluminescent methods, respectively. Consistently, under F806 treatment the AMP\u2010activated protein kinase signalling was activated, and autophagy flux was promoted and more autophagosomes were formed. Moreover, live\u2010cell imaging and immunofluorescence analysis showed that F806 induced GLUT1 plasma membrane dissociation and promoted its internalization and autolysosome accumulation and lysosome degradation. Furthermore, molecular docking studies demonstrated that F806 bound to GLUT1 with a comparable binding energy to that of GLUT1\u2019s direct interacting inhibitor cytochalasin B. Amino acid mutation was used to test which residues of GLUT1 may participate in F806 mediated\u2010GLUT1 internalization and degradation, and results showed that Thr137, Asn411 and Trp388 were required for GLUT1 internalization and degradation, respectively. Taken together, these findings shed light on a novel anti\u2010tumour mechanism of F806 by targeting and promoting GLUT1 internalization and further autolysosomal degradation.<\/jats:p>","DOI":"10.1111\/febs.16545","type":"journal-article","created":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T16:44:28Z","timestamp":1654188268000},"page":"6782-6798","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Anti\u2010tumour effects of a macrolide analog <scp>F806<\/scp> in oesophageal squamous cell carcinoma cells by targeting and promoting <scp>GLUT1<\/scp> autolysosomal degradation"],"prefix":"10.1111","volume":"289","author":[{"given":"Xiang","family":"Li","sequence":"first","affiliation":[{"name":"The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Department of Biochemistry and Molecular Biology Shantou University Medical College  China"},{"name":"Cancer Research Center Shantou University Medical College  China"},{"name":"Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Institute of Oncologic Pathology Shantou University Medical College  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liyan","family":"Li","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Department of Biochemistry and Molecular Biology Shantou University Medical College  China"},{"name":"Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Institute of Oncologic Pathology Shantou University Medical College  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodong","family":"Wu","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Department of Biochemistry and Molecular Biology Shantou University Medical College  China"},{"name":"Medical Informatics Research Center Shantou University Medical College  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Wen","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Department of Biochemistry and Molecular Biology Shantou University Medical College  China"},{"name":"Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Institute of Oncologic Pathology Shantou University Medical College  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wan","family":"Lin","sequence":"additional","affiliation":[{"name":"Cancer Research Center Shantou University Medical College  China"},{"name":"Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Institute of Oncologic Pathology Shantou University Medical College  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yufei","family":"Cao","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Department of Biochemistry and Molecular Biology Shantou University Medical College  China"},{"name":"Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Institute of Oncologic Pathology Shantou University Medical College  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Xie","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Department of Biochemistry and Molecular Biology Shantou University Medical College  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hefeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Key Laboratory for Preparation and Application of Ordered Structural Materials of Guangdong Province Shantou University  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geng","family":"Dong","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Department of Biochemistry and Molecular Biology Shantou University Medical College  China"},{"name":"Medical Informatics Research Center Shantou University Medical College  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6375-3614","authenticated-orcid":false,"given":"Enmin","family":"Li","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Department of Biochemistry and Molecular Biology Shantou University Medical College  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1618-4292","authenticated-orcid":false,"given":"Liyan","family":"Xu","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Department of Biochemistry and Molecular Biology Shantou University Medical College  China"},{"name":"Cancer Research Center Shantou University Medical College  China"},{"name":"Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Institute of Oncologic Pathology Shantou University Medical College  China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9256-103X","authenticated-orcid":false,"given":"Yinwei","family":"Cheng","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Department of Biochemistry and Molecular Biology Shantou University Medical College  China"},{"name":"Cancer Research Center Shantou University Medical College  China"},{"name":"Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Institute of Oncologic Pathology Shantou University Medical College  China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2012.07.001"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.08.021"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.02.013"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2015.10.007"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20133374"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40134-013-0016-x"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13306"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1603735113"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00387.2012"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.759175"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-12-0131"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24112159"},{"key":"e_1_2_10_14_1","first-page":"502","article-title":"Structure identification and anti\u2010tumor activity research of FW\u201004\u2010806","volume":"36","author":"Wei HWJ","year":"2011","journal-title":"Chin J Antibiot"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-13-150"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.3612"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2015.01.016"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9CC07363A"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.95"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.21.5720"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-020-01239-w"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.028"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2009.00894.x"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2013.01.035"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20081422"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.0c01054"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2017.05.020"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14577"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2014.10.040"},{"key":"e_1_2_10_30_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2018\/2049313","article-title":"F806 Suppresses the invasion and metastasis of esophageal squamous cell carcinoma via downregulating F\u2010actin assembly\u2010related rho family proteins","volume":"19","author":"Xie L","year":"2018","journal-title":"Biomed Res Int"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.12.016"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2008.03.003"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)89099-8"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2910861"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3020355"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.3359"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09234-6"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21256"},{"key":"e_1_2_10_39_1","volume-title":"AMBER 2018","author":"Case DA","year":"2018"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.22102"}],"container-title":["The FEBS Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.16545","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/febs.16545","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.16545","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T07:02:09Z","timestamp":1692687729000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/febs.16545"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,13]]},"references-count":39,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.1111\/febs.16545"],"URL":"https:\/\/doi.org\/10.1111\/febs.16545","archive":["Portico"],"relation":{},"ISSN":["1742-464X","1742-4658"],"issn-type":[{"value":"1742-464X","type":"print"},{"value":"1742-4658","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,13]]},"assertion":[{"value":"2022-01-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}