{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:19:36Z","timestamp":1791335976556,"version":"4.3.1"},"reference-count":62,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T00:00:00Z","timestamp":1664236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81770638"],"award-info":[{"award-number":["81770638"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81972300"],"award-info":[{"award-number":["81972300"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:p>\n                    <jats:italic>Fusobacterium nucleatum<\/jats:italic>\n                    (\n                    <jats:italic>Fn<\/jats:italic>\n                    ) is reportedly involved in poor prognosis of oesophageal squamous cell carcinoma (ESCC), but the responsible mechanisms remain unclear. The present study aimed to explore the function of\n                    <jats:italic>Fn<\/jats:italic>\n                    in ESCC progression, and to identify the key genes or signals involved. Fluorescence\n                    <jats:italic>in\u2009situ<\/jats:italic>\n                    hybridization and quantitative PCR assays were applied to measure the abundance of\n                    <jats:italic>Fn<\/jats:italic>\n                    in ESCC tissues, finding that ESCC tissues displayed a higher abundance of\n                    <jats:italic>Fn<\/jats:italic>\n                    compared to adjacent tissues. Furthermore,\n                    <jats:italic>Fn<\/jats:italic>\n                    abundance in advanced ESCC tissues was found to be higher than that in early stage ESCC. The proliferation assays and wound healing assays indicated that\n                    <jats:italic>Fn<\/jats:italic>\n                    infection promoted ESCC cell proliferation and migration. Based on high\u2010throughput sequencing, cytochrome P450 1A1 (CYP1A1) was the most significantly upregulated (eightfold increase) gene, and AKT signalling was activated in KYSE\u2010450 cells treated with\n                    <jats:italic>Fn<\/jats:italic>\n                    . Knocking down CYP1A1 or inactivating AKT signalling with LY294002 downregulated p\u2010AKT\n                    <jats:sup>S473<\/jats:sup>\n                    , inhibited cell proliferation, and compromised the proliferation effect induced by\n                    <jats:italic>Fn<\/jats:italic>\n                    in both\n                    <jats:italic>in\u2009vitro<\/jats:italic>\n                    and\n                    <jats:italic>in\u2009vivo<\/jats:italic>\n                    experiments. Inactivating the aryl hydrocarbon receptor (AHR) by CH\u2010223191 reversed CYP1A1 expression induced by\n                    <jats:italic>Fn<\/jats:italic>\n                    and inhibited the proliferation of ESCC cells. Taken together, our findings indicate that\n                    <jats:italic>Fn<\/jats:italic>\n                    may promote ESCC cell proliferation via AHR\/CYP1A1\/AKT signalling. Targeting\n                    <jats:italic>Fn<\/jats:italic>\n                    or AHR\/CYP1A1 signalling could yield approaches relevant to the treatment of ESCC.\n                  <\/jats:p>","DOI":"10.1111\/febs.16619","type":"journal-article","created":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T10:29:54Z","timestamp":1662546594000},"page":"837-854","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["<i>Fusobacterium nucleatum<\/i>\n                    promotes proliferation in oesophageal squamous cell carcinoma via\n                    <scp>AHR<\/scp>\n                    \/\n                    <scp>CYP1A1<\/scp>\n                    signalling"],"prefix":"10.1111","volume":"290","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8500-6468","authenticated-orcid":false,"given":"Haisen","family":"Yin","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Renmin Hospital of Wuhan 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