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Using quantitative proteomics, it was recently found that FKBP25 was expressed in the top 10% of the mouse skeletal muscle proteome. However, to date there have been few studies investigating the role of\u00a0FKBP25 in non\u2010transformed systems. As such, this study aimed to investigate potential roles for FKBP25 in myoblast viability, migration and differentiation and in adaptation of mature skeletal muscle. Doxycycline\u2010inducible FKBP25 knockdown in C2C12 myoblasts revealed an increase in cell accumulation\/viability and migration <jats:italic>in vitro<\/jats:italic> that was independent of alterations in tubulin dynamics; however, FKBP25 knockdown had no discernible impact on myoblast differentiation into myotubes. Finally, a series of <jats:italic>in vivo<\/jats:italic> models of muscle adaptation were assessed, where it was observed that FKBP25 protein expression was increased in hypertrophy and regeneration conditions (chronic mechanical overload and the <jats:italic>mdx<\/jats:italic> model of Duchenne muscular dystrophy) but decreased in an atrophy model (denervation). Overall, the findings of this study establish FKBP25 as a regulator of myoblast viability and migration, with possible implications for satellite cell proliferation and migration and muscle regeneration, and as a potential regulator of <jats:italic>in vivo<\/jats:italic> skeletal muscle adaptation.<\/jats:p>","DOI":"10.1111\/febs.16894","type":"journal-article","created":{"date-parts":[[2023,6,24]],"date-time":"2023-06-24T10:42:27Z","timestamp":1687603347000},"page":"4660-4678","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>FKBP25<\/scp> regulates myoblast viability and migration and is differentially expressed in <i>in vivo<\/i> models of muscle adaptation"],"prefix":"10.1111","volume":"290","author":[{"given":"Tabitha","family":"Cree","sequence":"first","affiliation":[{"name":"Institute for Health and Sport (IHeS) Victoria University  Melbourne Australia"},{"name":"Australian Institute for Musculoskeletal 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