{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T23:43:33Z","timestamp":1788997413838,"version":"build-2803163510"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T00:00:00Z","timestamp":1697414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31671564"],"award-info":[{"award-number":["31671564"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31970797"],"award-info":[{"award-number":["31970797"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271720"],"award-info":[{"award-number":["82271720"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>\n                    Decidualization of endometrial stroma is a key step in embryo implantation and its abnormality often leads to pregnancy failure. Stromal decidualization is a very complex process that is co\u2010regulated by estrogen, progesterone and many local factors. The signaling protein SHP2 encoded by\n                    <jats:italic>PTPN11<\/jats:italic>\n                    is dynamically expressed in decidualized endometrial stroma and mediates and integrates various signals to govern the decidualization. In the present study, we investigate the mechanism of\n                    <jats:italic>PTPN11<\/jats:italic>\n                    gene transcription. Estrogen, progesterone and cAMP co\u2010induced decidualization of human endometrial stromal cell\n                    <jats:italic>in vitro<\/jats:italic>\n                    , but only progesterone and cAMP induced SHP2 expression. Using the luciferase reporter, we refined a region from \u2212229\u2009bp to +1\u2009bp in the\n                    <jats:italic>PTPN11<\/jats:italic>\n                    gene promoter comprising the transcriptional core regions that respond to progesterone and cAMP. Progesterone receptor (PGR) and cAMP\u2010responsive element\u2010binding protein 1 (CREB1) were predicted to be transcription factors in this core region by bioinformatic methods. The direct binding of PGR and CREB1 on the\n                    <jats:italic>PTPN11<\/jats:italic>\n                    promoter was confirmed by electrophoretic mobility and chromatin immunoprecipitation\n                    <jats:italic>in\u2009vitro<\/jats:italic>\n                    . Knockdown of PGR and CREB1 protein significantly inhibited the expression of SHP2 induced by medroxyprogesterone acetate and cAMP. These results demonstrate that transcription factors PGR and CREB1 bind to the\n                    <jats:italic>PTPN11<\/jats:italic>\n                    promoter to regulate the expression of SHP2 in response to decidual signals. Our results explain the transcriptional expression mechanism of SHP2 during decidualization and promote the understanding of the mechanism of decidualization of stromal cells.\n                  <\/jats:p>","DOI":"10.1111\/febs.16966","type":"journal-article","created":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T03:31:10Z","timestamp":1696303870000},"page":"142-157","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Progesterone and cAMP synergistically induce SHP2 expression via PGR and CREB1 during uterine stromal decidualization"],"prefix":"10.1111","volume":"291","author":[{"given":"Peiyi","family":"Zhou","sequence":"first","affiliation":[{"name":"Xiamen City Key Laboratory of Metabolism, School of Pharmaceutical Sciences Xiamen University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqun","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Xiamen City Key Laboratory of Metabolism, School of Pharmaceutical Sciences Xiamen University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Jiang","sequence":"additional","affiliation":[{"name":"Xiamen City Key Laboratory of Metabolism, School of Pharmaceutical Sciences Xiamen University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingpu","family":"Tian","sequence":"additional","affiliation":[{"name":"Xiamen City Key Laboratory of Metabolism, School of Pharmaceutical Sciences Xiamen University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Deng","sequence":"additional","affiliation":[{"name":"Reproductive Medical Centre The First Affiliated Hospital of Xiamen University  China"},{"name":"Fujian Provincial Key Laboratory of Reproductive Health Research Medical College of Xiamen University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibin","family":"Wang","sequence":"additional","affiliation":[{"name":"Reproductive Medical Centre The First Affiliated Hospital of Xiamen University  China"},{"name":"Fujian Provincial Key Laboratory of Reproductive Health Research Medical College of Xiamen University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7513-4041","authenticated-orcid":false,"given":"Shuangbo","family":"Kong","sequence":"additional","affiliation":[{"name":"Reproductive Medical Centre The First Affiliated Hospital of Xiamen University  China"},{"name":"Fujian Provincial Key Laboratory of Reproductive Health Research Medical College of Xiamen University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1682-7147","authenticated-orcid":false,"given":"Zhongxian","family":"Lu","sequence":"additional","affiliation":[{"name":"Xiamen City Key Laboratory of Metabolism, School of Pharmaceutical Sciences Xiamen University  China"},{"name":"Fujian Provincial Key Laboratory of Reproductive Health Research Medical College of Xiamen University  China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)00682-6"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2012.12.011"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21114092"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12473"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10-175349"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend.14.8.0498"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00342-07"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2012-1585"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-08-0539"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.3.1032"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00066.2003"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3012"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0029-1242989"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1808"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568009614666140717105001"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1010018"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1700978114"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8101272"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2019.104595"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep12982"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1572"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.4103\/aja.aja_49_19"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2645"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2015.04.012"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1156"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2006.12.011"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0024-8"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/2211-5463.12992"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.10.1.135"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2020.112117"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2015-1274"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.2037511"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(97)00178-0"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2014-1566"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11247"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2014-1045"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-22-0367"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2864-16.2017"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(90)90115-V"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1780357"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.23569"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2013.02.001"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/1043-2760(95)00204-9"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmb2.12088"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02820507"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2014.02.005"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2017.5636"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2017.7654"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes11080923"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.116979"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioab077"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/asj.12156"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gah186"}],"container-title":["The FEBS Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/febs.16966","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T05:19:34Z","timestamp":1705555174000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/febs.16966"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,16]]},"references-count":53,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1111\/febs.16966"],"URL":"https:\/\/doi.org\/10.1111\/febs.16966","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/FEBS.16966\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/FEBS.16966\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/FEBS.16966\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/FEBS.16966\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/FEBS.16966\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/FEBS.16966\/v2\/review1","asserted-by":"object"}]},"ISSN":["1742-464X","1742-4658"],"issn-type":[{"value":"1742-464X","type":"print"},{"value":"1742-4658","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,16]]},"assertion":[{"value":"2023-05-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}