{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T11:45:24Z","timestamp":1782215124810,"version":"3.54.5"},"reference-count":49,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,12,8]],"date-time":"2023-12-08T00:00:00Z","timestamp":1701993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2023,12,8]],"date-time":"2023-12-08T00:00:00Z","timestamp":1701993600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2019YFA0802003"],"award-info":[{"award-number":["2019YFA0802003"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170300"],"award-info":[{"award-number":["82170300"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:p>\n                    The scaffolding protein programmed cell death protein 10 (Pdcd10) has been demonstrated to play a critical role in renal epithelial cell homeostasis and function by maintaining appropriate water reabsorption in collecting ducts. Both ureter and kidney collecting duct systems are derived from the ureter bud during development. Here, we report that cadherin\u201016 (\n                    <jats:italic>Cdh16<\/jats:italic>\n                    )\u2010cre drives gene recombination with high specificity in the ureter, but not the bladder, urothelium. The consequences of\n                    <jats:italic>Pdcd10<\/jats:italic>\n                    deletion on the stratified ureter urothelium were investigated using an integrated approach including messenger RNA (mRNA) expression analysis, immunocytochemistry, and high\u2010resolution confocal and electron microscopy. Loss of\n                    <jats:italic>Pdcd10<\/jats:italic>\n                    in the ureter urothelium resulted in increased expression of uroplakins (Upks) and keratins (Krts), as well as hypertrophy of the ureter urothelium with an associated increase in the number of proliferation marker protein Ki\u201067 (\n                    <jats:italic>Ki67<\/jats:italic>\n                    )\u2010expressing cells specifically within the basal urothelium layer. Ultrastructural analysis documented significant modification of the intracellular membrane system, including intracellular vesicle genesis and transport along the basal\u2010 to umbrella\u2010cell\u2010layer axis. Additionally,\n                    <jats:italic>Pdcd10<\/jats:italic>\n                    loss resulted in swelling of Golgi compartments, disruption of mitochondrial cristae structure, and increased lysosomal fusion. Lack of\n                    <jats:italic>Pdcd10<\/jats:italic>\n                    also resulted in decreased fusiform vesicle formation in umbrella cells, increased secretion of exosome vesicles, and alteration in microvillar structure on apical membranes. Our findings indicate that\n                    <jats:italic>Pdcd10<\/jats:italic>\n                    expression and its influence on homeostasis is associated with modulation of endomembrane trafficking and organelle biogenesis in the ureter urothelium.\n                  <\/jats:p>","DOI":"10.1111\/febs.17022","type":"journal-article","created":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T02:09:11Z","timestamp":1701396551000},"page":"1008-1026","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Deficiency of Pdcd10 causes urothelium hypertrophy and vesicle trafficking defects in ureter"],"prefix":"10.1111","volume":"291","author":[{"given":"Yixuan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Tianjin Key Laboratory of Inflammation Biology, School of Basic Medical Sciences, and Center for Cardiovascular Diseases Tianjin Medical University  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