{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T21:51:12Z","timestamp":1789681872610,"version":"4.0.1"},"reference-count":33,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T00:00:00Z","timestamp":1714003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2021A1515010003"],"award-info":[{"award-number":["2021A1515010003"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2021A1515011705"],"award-info":[{"award-number":["2021A1515011705"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81972319"],"award-info":[{"award-number":["81972319"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82002555"],"award-info":[{"award-number":["82002555"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173187"],"award-info":[{"award-number":["82173187"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["febs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FEBS Journal"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:p>\n                    Immune checkpoint inhibitors provide a definite survival benefit for patients with driver\u2010negative advanced non\u2010small cell lung cancer (NSCLC), but predictors of efficacy are still lacking. There may be a relationship between immune inflammatory state and tumor immune response. We explored the relationship of serum neutrophil extracellular traps (NETs) with infiltrating cells in the tumor tissues of patients with NSCLC as well as their relationship with the therapeutic efficacy of programmed cell death protein 1 (PD\u20101) inhibitors. Serum myeloperoxidase (MPO)\u2010double\u2010stranded DNA (dsDNA) was detected as a marker of NET serum concentration. T\u00a0cells were detected by immunohistochemical staining, and neutrophils were counted by MPO immunofluorescence staining. Of the 31 patients with NSCLC, a longer progression\u2010free survival after PD\u20101 inhibitor treatment was associated with higher levels of CD3\n                    <jats:sup>+<\/jats:sup>\n                    T\u00a0cells, a lower neutrophil\u2009:\u2009CD3\n                    <jats:sup>+<\/jats:sup>\n                    \u2010T\u2010cell ratio (NEU\/CD3\n                    <jats:sup>+<\/jats:sup>\n                    ) and lower neutrophil\u2009: CD8\n                    <jats:sup>+<\/jats:sup>\n                    \u2010T\u2010cell ratio (NEU\/CD8\n                    <jats:sup>+<\/jats:sup>\n                    ) in tumor tissues. Patients with higher serum NETs were more likely to develop progressive disease after treatment (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.003) and to have immune\u2010related adverse events (IrAEs) as well as higher NEU\/CD3\n                    <jats:sup>+<\/jats:sup>\n                    and NEU\/CD8\n                    <jats:sup>+<\/jats:sup>\n                    . The combined model of serum NETs, CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u00a0cells, and tumor proportion score (TPS) significantly improved the prediction of PD\u20101 inhibitor efficacy [\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.033; area under the curve (AUC)\u2009=\u20090.881]. Our results indicate that serum NETs are effective predictors of PD\u20101 inhibitor response and reflect the tissue neutrophil\u2010to\u2010lymphocyte ratio and IrAE levels. The combined model of serum NETs, CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u00a0cells, and TPS is a powerful tool for predicting the efficacy of PD\u20101 inhibitor treatment in patients with NSCLC.\n                  <\/jats:p>","DOI":"10.1111\/febs.17144","type":"journal-article","created":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T10:35:21Z","timestamp":1714041321000},"page":"3403-3416","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["A combined model of serum neutrophil extracellular traps,\n                    <scp>CD<\/scp>\n                    8\n                    <sup>+<\/sup>\n                    T\u00a0cells, and tumor proportion score provides better prediction of\n                    <scp>PD<\/scp>\n                    \u20101 inhibitor efficacy in patients with\n                    <scp>NSCLC<\/scp>"],"prefix":"10.1111","volume":"291","author":[{"given":"Jian","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Oncology, Nanfang Hospital Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Shu","sequence":"additional","affiliation":[{"name":"Department of Oncology, Nanfang Hospital Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Oncology, Nanfang Hospital Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Oncology, Nanfang Hospital Southern Medical University  Guangzhou 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