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This change leads to a less compact structure, characterized by an increased presence of solvent\u2010exposed hydrophobic patches and \u03b2\u2010sheet\u2010rich regions. This transformation is validated by circular dichroism, thioflavin T binding, and Bis\u2010ANS assays. The formation of this \u03b2\u2010sheet\u2010rich conformation, which is amplified in the absence of zinc, leads to protein aggregation. This aggregation is induced not only by high temperatures but also by low ionic strength and high protein concentration. The aggregated conformation exhibits characteristics of an amyloidogenic structure, including a distinctive X\u2010ray diffraction pattern, seeding competence (which stimulates the formation of amyloid\u2010like aggregates), cytotoxicity, resistance to SDS, and fibril formation. 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Our discovery of amyloid aggregation in a C\u2010terminal deletion mutant of DNAJA1, which resembles a spliced homolog expressed in the testis, implies that Type I Hsp40 co\u2010chaperones may generate amyloidogenic species\n                    <jats:italic>in vivo<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/febs.17215","type":"journal-article","created":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T09:51:13Z","timestamp":1720432273000},"page":"3904-3923","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Type I Hsp40s\/\n                    <scp>DnaJs<\/scp>\n                    aggregates exhibit features reminiscent of amyloidogenic structures"],"prefix":"10.1111","volume":"291","author":[{"given":"Ana O.","family":"Tiroli\u2010Cepeda","sequence":"first","affiliation":[{"name":"Institute of Chemistry, Universidade Estadual de Campinas\u2010UNICAMP  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo A.","family":"Linhares","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, Universidade Estadual de Campinas\u2010UNICAMP  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annelize Z. 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