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Its inactivation has been closely associated with leukemia diseases. However, the physiological function of PP2A for hematopoiesis has been poorly understood in organisms.\n                    <jats:italic>Drosophila<\/jats:italic>\n                    hematopoiesis parallels the vertebrate counterpart in developmental and functional features but involves a much simpler hematopoietic system. Here, utilizing the\n                    <jats:italic>Drosophila<\/jats:italic>\n                    major larval hematopoietic organ lymph gland, we studied the function of PP2A for hematopoiesis\n                    <jats:italic>in\u2009vivo<\/jats:italic>\n                    . By knocking down the expression of\n                    <jats:italic>Pp2A\u201029B<\/jats:italic>\n                    that encodes the scaffold subunit of the PP2A holoenzyme complex, we found that PP2A silencing in the differentiating hemocytes resulted in their excessive proliferation. Furthermore, this PP2A inhibition downregulated the expression of Smoothened (Smo), a crucial component in the Hedgehog pathway, and\n                    <jats:italic>smo<\/jats:italic>\n                    overexpression was able to rescue the phenotypes of PP2A depletion, indicating that Smo functions as a downstream effector of PP2A to restrict the hemocyte proliferation. PDGF\/VEGF\u2010receptor (Pvr) overexpression also restored the Smo expression and lymph gland morphology of PP2A silencing, suggesting a PP2A\u2010Pvr\u2010Smo axis to regulate lymph gland growth and hemocyte proliferation. Moreover, inhibiting PP2A activity in the blood progenitor cells promoted their differentiation, but which was independent with Smo. Together, our data suggested that PP2A plays a dual role in the\n                    <jats:italic>Drosophila<\/jats:italic>\n                    lymph gland by preserving the progenitor population and restraining the hemocyte proliferation, to properly regulate the hematopoietic process.\n                  <\/jats:p>","DOI":"10.1111\/febs.17247","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T06:46:12Z","timestamp":1724654772000},"page":"4558-4580","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Protein phosphatase 2\n                    <scp>A<\/scp>\n                    regulates blood cell proliferation and differentiation in\n                    <i>Drosophila<\/i>\n                    larval lymph glands"],"prefix":"10.1111","volume":"291","author":[{"given":"Fang","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Evolution &amp; Marine Biodiversity (Ministry of Education) and Institute of Evolution &amp; Marine Biodiversity Ocean University of China  Qingdao China"},{"name":"College of Marine Life Sciences Ocean University of China  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0013-1409","authenticated-orcid":false,"given":"Wang","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Evolution &amp; Marine Biodiversity (Ministry of Education) and Institute of Evolution &amp; Marine Biodiversity Ocean University of China  Qingdao China"},{"name":"College of Marine Life Sciences Ocean University of China  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumin","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Evolution &amp; Marine Biodiversity (Ministry of Education) and Institute of Evolution &amp; Marine Biodiversity Ocean University of China  Qingdao 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